Fine-mapping loss of gene architecture at the CDKN2B (p15INK4b), CDKN2A (p14ARF, p16INK4a), and MTAP genes in head and neck squamous cell carcinoma

Archives of Otolaryngology--head & Neck Surgery
Maria J WorshamMichael S Benninger

Abstract

To identify the extent and the smallest region of loss for CDKN2B(INK4b), CDKN2A(ARF,INK4a), and MTAP. Homozygous deletions of human chromosome 9p21 occur frequently in malignant cell lines and are common in squamous cell carcinoma of the head and neck (HNSCC). This complex region encodes the tumor suppressor genes cyclin-dependent kinase 2B (CDKN2B) (p15(INK4b)) and CDKN2A (p14(ARF), p16(INK4a)) and the housekeeping gene methylthioadenosine phosphorylase (MTAP). A targeted probe panel designed to finely map the region of 9p21 loss comprised 3 probes for CDKN2B(INK4b), 7 for CDKN2A(ARF, INK4a), and 3 for MTAP and was interrogated using the multiplex ligation-dependent probe amplification assay (MLPA). The MLPA genomic copy number alterations for CDKN2A were validated using real-time polymerase chain reaction. Six HNSCC primary (A) and recurrent or metastatic (B) cell lines were examined: UMSCC-11A/11B, UMSCC-17A/17B, and UMSCC-81A/81B. Cell line UMSCC-11B retained all 9p loci tested in the region. Cell lines UMSCC-17A/B indicated homozygous deletion of CDKN2A(ARF, INK4a) starting at p16(INK4) exon 1alpha to include exons 2 and 3. Homozygous loss was indicated for CDKN2B(INK4b) and CDKN2A(ARF,INK4a) in UMSCC-11A, and UMSCC-81A. ...Continue Reading

Citations

May 14, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Bhavna KumarThomas E Carey
Dec 3, 2008·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Josena K StephenMaria J Worsham
May 12, 2009·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Seema SethiMaria J Worsham
Jul 14, 2010·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Maria J WorshamMichael S Benninger
Mar 14, 2012·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Maria J WorshamVanessa P Schweitzer
May 10, 2012·Journal of Oncology·Maria J WorshamVanessa P Schweitzer
Sep 21, 2011·Expert Review of Anticancer Therapy·Maria J Worsham
Dec 23, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Shinsuke OhtaYoshinobu Kubota
Sep 18, 2009·The Journal of Investigative Dermatology·Rocío SalgadoBlanca Espinet
Jun 19, 2008·Cancer Genetics and Cytogenetics·Maciej GiefingKrzysztof Szyfter
Jul 16, 2010·Head & Neck·César Álvarez MarcosJosé Luis Llorente
Dec 16, 2011·Journal of Biophotonics·Thomas Dreyer, Melanie Kreisel
Dec 19, 2013·Statistics in Medicine·Jichen YangGuanghua Xiao
Mar 24, 2016·Cancer Biomarkers : Section a of Disease Markers·Ahmed MahasMichael Markey
Aug 7, 2012·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Blanca R Ibieta-ZarcoMarcela Lizano
Jul 6, 2016·Pathology Oncology Research : POR·Fernando LópezCésar Álvarez-Marcos
Oct 4, 2016·Head & Neck·Zubeyde YalnizNejat Dalay
Aug 4, 2007·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Josena K StephenMaria J Worsham
Nov 29, 2007·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Ozlen SaglamMaria J Worsham
Feb 12, 2011·Journal of Neuro-oncology·Fateme SalehiRicardo V Lloyd
Jun 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Reika IwakawaJun Yokota
May 24, 2011·International Journal of Head and Neck Surgery·Josena K StephenMaria J Worsham
Mar 6, 2012·Cancer Letters·Maria J WorshamVanessa G Schweitzer

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