Fine-needle aspiration of adult small-round-cell tumors studied with flow cytometry

Diagnostic Cytopathology
Marino E LeonFernando U Garcia

Abstract

Immunophenotypic study is critical for the diagnosis of adult small-round-cell tumors (SRCTs). We describe three patients with Ewing's sarcoma/primitive neuroectodermal tumor (ES/PNET) and one patient with neuroblastoma in which flow cytometry immunophenotyping (FCI) on the fine-needle aspirate (FNA) and bone marrow aspirate (BMA) demonstrated an abnormal population of cells that were CD45(-) and CD16/CD56(+). Four patients with mean age of 30 years, three male and one female, clinically suspicious for a lymphoma or SRCT are described. FNA, BMA, and biopsy specimens were obtained for routine cytologic and histologic evaluation. Fresh tissue was studied by FCI. In all cases, the cytology smears showed small cells with round nuclei, slightly irregular nuclear membranes, fine chromatin, and scant cytoplasm. FCI showed CD16/56(+) and CD45(-) neoplastic cells in all cases. In one case, 76% of these cells were CD99(+). The diagnoses of ES/PNET were confirmed by immunohistochemical, ultrastructural, and cytogenetic studies. ES/PNET in FNA and BMA can be efficiently and rapidly diagnosed by combining cytologic examination with FCI using a panel including CD45, CD16/56, and CD99.

References

Jan 1, 1992·Diagnostic Cytopathology·A M GurleyV V Joshi
Jan 1, 1991·Diagnostic Cytopathology·L J LayfieldC P Reynolds
Jun 1, 1989·The Journal of Experimental Medicine·L L LanierJ H Phillips
Jan 1, 1993·Human Pathology·M Miettinen, W Cupo
Dec 1, 1993·Cancer Genetics and Cytogenetics·L SainatiG Basso
Jan 1, 1996·Urologia Internationalis·J H MydloR J Macchia
Nov 1, 1996·American Journal of Clinical Pathology·A A RenshawS R Granter
Dec 1, 1996·Human Pathology·S R GranterA E Rosenberg
Mar 21, 1998·The American Journal of Surgical Pathology·M MiettinenA Stevenson
Aug 6, 1998·Virchows Archiv : an International Journal of Pathology·L J GardnerC H Dunphy
Dec 23, 1998·Cytopathology : Official Journal of the British Society for Clinical Cytology·S AtahanC Ekinci
May 13, 1999·Diagnostic Cytopathology·L J LayfieldR K Dodge
Dec 10, 1999·American Journal of Clinical Pathology·S IravaniB Schnitzer
Apr 29, 2000·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·P J ZhangJ J Brooks
Jun 2, 2000·Acta Cytologica·K SahuU N Khadilkar
Jun 2, 2000·Acta Cytologica·R González-Cámpora
Feb 24, 2001·American Journal of Clinical Pathology·D R LucasM P Mott
Nov 6, 2001·Annales de génétique·A M UdayakumarL Appaji
Jul 9, 2002·Journal of Clinical Pathology·G J BrysonR C W Wong
Dec 24, 2002·Cytometry·Michael J WarzynskiYuki A Hammers

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Citations

Jan 1, 2009·Journal of Cytology·Arvind RajwanshiGautam Upasana
Dec 30, 2014·Indian Journal of Medical and Paediatric Oncology : Official Journal of Indian Society of Medical & Paediatric Oncology·Cherry BansalAnand Srivastava
Aug 28, 2009·Pediatric Blood & Cancer·Steven G DuboisElizabeth Sinclair
Feb 11, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kieuhoa T VoSteven G Dubois
Apr 7, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shifra AshSmadar Avigad

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