Fine scale analysis of crossover and non-crossover and detection of recombination sequence motifs in the honeybee (Apis mellifera).

PloS One
Nadia BessoltaneFlorence Mougel

Abstract

Meiotic exchanges are non-uniformly distributed across the genome of most studied organisms. This uneven distribution suggests that recombination is initiated by specific signals and/or regulations. Some of these signals were recently identified in humans and mice. However, it is unclear whether or not sequence signals are also involved in chromosomal recombination of insects. We analyzed recombination frequencies in the honeybee, in which genome sequencing provided a large amount of SNPs spread over the entire set of chromosomes. As the genome sequences were obtained from a pool of haploid males, which were the progeny of a single queen, an oocyte method (study of recombination on haploid males that develop from unfertilized eggs and hence are the direct reflect of female gametes haplotypes) was developed to detect recombined pairs of SNP sites. Sequences were further compared between recombinant and non-recombinant fragments to detect recombination-specific motifs. Recombination events between adjacent SNP sites were detected at an average distance of 92 bp and revealed the existence of high rates of recombination events. This study also shows the presence of conversion without crossover (i. e. non-crossover) events, the numb...Continue Reading

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Citations

Oct 29, 2013·Molecular Genetics and Genomics : MGG·Florence MougelMichel Solignac
Aug 21, 2012·Nucleic Acids Research·Wayne P Wahls, Mari K Davidson
Jun 12, 2013·Communicative & Integrative Biology·Clement F Kent, Amro Zayed
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Clement F KentAmro Zayed
Dec 4, 2016·Annual Review of Entomology·Amy L Toth, Sandra M Rehan
Jun 1, 2016·Bulletin of Entomological Research·S Łopuch, A Tofilski
Aug 6, 2016·Genome Biology and Evolution·Andrew B AdrianJosep M Comeron
Jun 10, 2021·Proceedings. Biological Sciences·Benjamin P OldroydMadeleine Beekman

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Methods Mentioned

BETA
Whole Genome Shotgun sequencing

Software Mentioned

DIALIGN2
RepeatMasker
sig
R
Megablast
analysis
- analysis tool
HGSC
Oligo
MEME

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