Fine-scale comparative genetic and physical mapping supports map-based cloning strategies for the self-incompatibility loci of perennial ryegrass (Lolium perenne L.).

Plant Molecular Biology
Hiroshi ShinozukaJohn W Forster

Abstract

Perennial ryegrass is an obligate outbreeding pasture grass of the Poaceae family, with a two-locus (S and Z) gametophytic self-incompatibility (SI) mechanism. This system has provided a major obstacle to targeted varietal development, and enhanced knowledge is expected to support more efficient breeding strategies. Comparative genetics and physical mapping approaches have been developed to permit molecular cloning of the SI genes. SI gene-linked genetic markers based on heterologous cDNA restriction fragment length polymorphisms (RFLPs) and homologous genomic DNA-derived simple sequence repeats (SSRs) were converted to single nucleotide polymorphism (SNP) format for efficient genotyping. Genetic mapping identified the location of SI loci and demonstrated macrosynteny between related grass species. S- and Z-linked bacterial artificial chromosome (BAC) clones were sequenced using massively parallel pyrosequencing technology to provide the first physical mapping data for Poaceae SI loci. The sequence assembly process suggested a lower prevalence of middle repetitive sequences in the Z locus region and hence precedence for positional cloning strategy. In silico mapping using data from rice, Brachypodium distachyon and Sorghum reve...Continue Reading

References

Dec 18, 2001·Plant Physiology·J DraperA P Routledge
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Frank B DeanRoger S Lasken
Apr 19, 2002·Genome Génome / Conseil National De Recherches Canada·Elizabeth S JonesJohn W Forster
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S. JonesW. Forster
Mar 21, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E Ortega, F Dicenta
May 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·L YanJ Dubcovsky
Oct 23, 2004·Genome Génome / Conseil National De Recherches Canada·X-Y BianP Langridge
Nov 5, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M J FavilleJ W Forster
Feb 18, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B Hackauf, P Wehling
May 3, 2005·Annual Review of Plant Biology·Seiji Takayama, Akira Isogai
Aug 16, 2005·Nature·UNKNOWN International Rice Genome Sequencing Project
Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·C Nathan HancockBruce A McClure
Jan 5, 2006·The New Phytologist·M W HumphreysL Skøt
May 19, 2006·Molecular Genetics and Genomics : MGG·Noel O I CoganJohn W Forster
Oct 3, 2006·The Plant Journal : for Cell and Molecular Biology·Etienne PauxCatherine Feuillet
Jan 6, 2007·Science·Ian ArmsteadIan King
May 31, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J M Alonso Segura, R Socias I Company
Mar 18, 2008·Trends in Plant Science·Magdalena OpanowiczJohn H Doonan
Apr 1, 2008·The New Phytologist·Bicheng YangSusanne Barth
May 1, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P M DracatosJ W Forster
May 17, 2008·International Journal of Plant Genomics·Andrew H Paterson

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Citations

May 1, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·P M DracatosJ W Forster
Nov 2, 2013·Journal of Experimental Botany·Ryan WhitfordPeter Langridge
Sep 15, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Steven R LarsonRichard R-C Wang
Dec 15, 2015·Molecular Biology and Evolution·Chloé ManzanaresBruno Studer
Sep 27, 2015·The Plant Journal : for Cell and Molecular Biology·Stephen L ByrneTorben Asp
Sep 1, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Javier Do CantoThomas Lubberstedt
Aug 26, 2017·Genome Génome / Conseil National De Recherches Canada·Hiroshi ShinozukaJohn W Forster
Jun 7, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Steve LarsonKevin Jensen
Apr 9, 2020·Frontiers in Plant Science·Juan Vicente Muñoz-SanzCarlos Romero
Sep 1, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Liyuan PanJunyi Gai
Aug 14, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jared CrainJesse Poland
Mar 5, 2021·The Plant Genome·Shuhao YuYanqi Wu
Apr 20, 2021·Frontiers in Plant Science·Xiaoping LianFengyi Hu
Mar 24, 2021·Annals of Botany·Claudio CropanoDaniel Thorogood
Nov 2, 2021·Frontiers in Plant Science·Claudio CropanoBruno Studer

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