PMID: 11903630Mar 21, 2002Paper

Fine scale mapping in the sex locus region of the honey bee (Apis mellifera)

Insect Molecular Biology
M HasselmannM Beye

Abstract

Isolating an unknown gene with fine-scale mapping is possible in a "non-model" organism. Sex determination in honey bees consists of a single locus (sex locus) with several complementary alleles. Diploid females are heterozygous at the sex locus, whereas haploid males arise from unfertilized eggs and are hemizygous. The construction of specific inbred crosses facilitates fine scale mapping in the sex locus region of the honey bee. The high recombination rate in the honey bee reduces the physical distance between markers compared with model organisms and facilitates a novel gene isolation strategy based on step-wise creation of new markers within small physical distances. We show that distances less than 25 kb can be efficiently mapped with a mapping population of only 1000 individuals. The procedure described here will accelerate the mapping, analysis and isolation of honey bee genes.

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Sep 1, 1994·Molecular & General Genetics : MGG·G J Hunt, R E Page
Jan 1, 1993·Annual Review of Immunology·J KleinN Takahata
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Mar 21, 2002·Insect Molecular Biology·M HasselmannM Beye

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Citations

Mar 26, 2003·Insect Biochemistry and Molecular Biology·M SumitaniM Hatakeyama
Oct 27, 2006·Genome Research·Martin BeyeRobert E Page
Sep 7, 2007·Annual Review of Entomology·George E Heimpel, Jetske G de Boer
Jul 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Martin HasselmannMartin Beye
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Martin Hasselmann, Martin Beye
Sep 24, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Martin Beye
Mar 20, 2015·PloS One·Leonela Carabajal PaladinoMaría José Bressa
May 17, 2006·Trends in Ecology & Evolution·Jay D EvansBenjamin P Oldroyd
May 17, 2006·Trends in Ecology & Evolution·Philip W HedrickRobert E Page
Mar 21, 2002·Insect Molecular Biology·M HasselmannM Beye
Feb 28, 2002·Genetics·Robert E PageMartin Beye

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