Fine-scale mapping of cortical laminar activity during sleep slow oscillations using high-density linear silicon probes

Journal of Neuroscience Methods
Richárd FiáthIstván Ulbert

Abstract

The cortical slow (∼1 Hz) oscillation (SO), which is thought to play an active role in the consolidation of memories, is a brain rhythm characteristic of slow-wave sleep, with alternating periods of neuronal activity and silence. Although the laminar distribution of cortical activity during SO is well-studied by using linear neural probes, traditional devices have a relatively low (20-100 μm) spatial resolution along cortical layers. In this work, we demonstrate a high-density linear silicon probe fabricated to record the SO with very high spatial resolution (∼6 μm), simultaneously from multiple cortical layers. Ketamine/xylazine-induced SO was acquired acutely from the neocortex of rats, followed by the examination of the high-resolution laminar structure of cortical activity. The probe provided high-quality extracellular recordings, and the obtained cortical laminar profiles of the SO were in good agreement with the literature data. Furthermore, we could record the simultaneous activity of 30-50 cortical single units. Spiking activity of these neurons showed layer-specific differences. The developed silicon probe measures neuronal activity with at least a three-fold higher spatial resolution compared with traditional linear p...Continue Reading

Citations

Apr 16, 2019·Frontiers in Neuroscience·Jacqueline van der MeijNiels C Rattenborg
May 16, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Gergely MártonKinga Tóth
Jul 17, 2021·Scientific Data·Csaba HorváthRichárd Fiáth

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