Fine specificity of T cell lines and clones that are capable of inducing autoimmune manifestations in mice

Cellular Immunology
S L KirshnerE Mozes

Abstract

Myasthenia gravis is a T-cell-regulated, antibody-mediated autoimmune disease. The synthetic peptides p195-212 and p259-271, which represent sequences of the human acetylcholine receptor alpha-subunit, preferentially stimulated T cells of patients with myasthenia gravis and were found to be immunodominant T cell epitopes in SJL and BALB/c mice, respectively. Therefore, we established a p195-212-specific T cell line from SJL mice and a p259-271-specific T cell line from BALB/c mice. N- and C-terminal truncated and/or extended peptides differed in their ability to stimulate proliferative responses of the lines and of their derived clones. Activated cells of the lines were inoculated into naive syngeneic mice. In both strains of mice, peptide-specific antibodies and antibodies to the murine acetylcholine receptor were detected. In addition, decremental compound muscle action potentials consistent with impairment of neuromuscular transmission were recorded from the line-inoculated mice. Thus, these T cell lines, clones, and epitopes constitute a useful model for investigating T cell pathogenicity in autoimmune manifestations related to myasthenia gravis.

Citations

Apr 13, 2006·Rejuvenation Research·Michael Sela
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Paas-RoznerE Mozes
Aug 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael Sela, Edna Mozes
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·A Faber-ElmannE Mozes
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y Katz-LevyE Mozes
Jul 16, 2005·Expert Opinion on Biological Therapy·Sonia Berrih-AkninMiriam C Souroujon
Dec 30, 1999·International Reviews of Immunology·M Sela
Mar 16, 2013·Nature Reviews. Drug Discovery·Guillaume VaquerBruno Sepodes

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