Fine structural ribonucleoprotein components of the cell nucleus visualized after spreading and high resolution autoradiography.

Chromosoma
S Fakan, M E Hughes

Abstract

The fine structure of the nuclear components was studied following mild lysis of mouse or Drosophila tissue culture cells and spreading of nuclear material. Particular attention was paid to nuclear ribonucleoprotein (RNP) constituents, which were analysed by high resolution autoradiography after [3H]uridine pulse labelling of cells. Comparison with the labelling kinetics of various in situ nuclear RNP constituents described previously suggests strong similarities between in situ constituents and structures observed within spread nuclear components. The present observations suggest that the nucleolar dense fibrillar component, shown previously in ultrathin sections of [3H]uridine-labelled intact cells as carrying rapidly labelled pre-rRNA, in fact consists of highly compacted transcribing ribosomal genes. The growing RNP fibrils appearing in transcription complexes of extranucleolar active genes and the in situ observed perichromatin fibrils also show the same labelling properties. This confirms that the two structures indeed represent the same nucleoplasmic constituents. As for the nuclear structures involved in post-transcriptional events, our observations demonstrate the occurrence of a rapidly labelled RNP fibro-granular net...Continue Reading

References

Aug 10, 1979·Chromosoma·M E HughesS Fakan
Sep 1, 1978·The Journal of Cell Biology·R HermanS Penman
Dec 1, 1975·Journal of Ultrastructure Research·R E NashW Bernhard
Jan 1, 1985·Biology of the Cell·M ThiryG Goessens
Mar 1, 1987·Cell Biology International Reports·S Ghosh, N Paweletz
Jan 1, 1972·Acta Endocrinologica. Supplementum·O L Miller, A H Bakken
May 1, 1969·Journal of Ultrastructure Research·A Monneron, W Bernhard
May 23, 1969·Science·O L Miller, B R Beatty
Jan 1, 1980·International Review of Cytology·S Fakan, E Puvion
Jan 1, 1984·International Review of Cytology·G Goessens
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·U Scheer, K M Rose

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Citations

Jul 1, 1991·Chromosoma·F Puvion-DutilleulE Puvion
Jan 1, 1990·Electron Microscopy Reviews·I RaskaL Salamin-Michel
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·M C Risueño, P S Testillano
Nov 1, 1992·Experimental Cell Research·P HozákD Hernandez-Verdun

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