PMID: 374986Jan 11, 1979Paper

Fine structure of the 21S ribosomal RNA region on yeast mitochondria DNA. I. Construction of the physical map and localization of the cistron for the 21S mitochondrial ribosomal RNA

Molecular & General Genetics : MGG
C HeytingP Borst

Abstract

1. We have used restriction enzyme analysis of petite mtDNAs to construct a detailed physical map of the 21S region on the mtDNA of the Saccharomyces cerevisiae strain JS1-3D. The map covers a segment of about 20,000 bp, on which the recognition sites of the enzymes HapII, HindII, HindIII, Sa1I, XhoI and HhaI have been localized (22 sites in total). This map has been checked in various ways against the independently constructed overall physical map of the mtDNA of strain JS1-3D. In addition, we have constructed a physical map with a resolution of about 200 bp of a HapII fragment of 1850 bp long, which carries the loci omega, RIB-1 and probably RIB-2. 2. The 21S rRNA hybridizes with the five adjacent HindII + III fragments TD9, DT19, TD15, DT14 and TT1, which lie in that order on the physical map of the 21S region. Of these, the two non-adjacent fragments TD9 and DT14 show a much stronger hybridization with 21S rRNA than DT19, TD15, and TT1. 3. The fragment DD5 (= DT19 + TD15) and part of DT14 belong to a sequence of about 1000 bp, which is absent from Saccharomyces carlsbergensis mtDNA. Although DD5 and DT14 show (very weak, respectively stronger) hybridization with 21S rRNA, the 1000 bp insert probably does not code for the 21...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S SatoJ I Harris
Jan 5, 1978·Journal of Molecular Biology·J R ArrandR J Roberts
Feb 25, 1975·Journal of Molecular Biology·R OldG Boizes
Mar 25, 1976·Journal of Molecular Biology·R J RobertsK Murray
Jul 28, 1970·Journal of Molecular Biology·T J Kelly, H O Smith

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Citations

Feb 1, 1980·Current Genetics·J LockerM Rabinowitz
Jan 22, 1982·Nucleic Acids Research·C Schmelzer, R J Schweyen

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