Fine-tuned ATP signals are acute mediators in osteocyte mechanotransduction

Cellular Signalling
Tina M KringelbachNiklas Rye Jørgensen

Abstract

Osteocytes are considered the primary mechanosensors of bone, but the signaling pathways they apply in mechanotransduction are still incompletely investigated and characterized. A growing body of data strongly indicates that P2 receptor signaling among osteoblasts and osteoclasts has regulatory effects on bone remodeling. Therefore, we hypothesized that ATP signaling is also applied by osteocytes in mechanotransduction. We applied a short fluid pulse on MLO-Y4 osteocyte-like cells during real-time detection of ATP and demonstrated that mechanical stimulation activates the acute release of ATP and that these acute ATP signals are fine-tuned according to the magnitude of loading. ATP release was then challenged by pharmacological inhibitors, which indicated a vesicular release pathway for acute ATP signals. Finally, we showed that osteocytes express functional P2X2 and P2X7 receptors and respond to even low concentrations of nucleotides by increasing intracellular calcium concentration. These results indicate that in osteocytes, vesicular ATP release is an acute mediator of mechanical signals and the magnitude of loading. These and previous results, therefore, implicate purinergic signaling as an early signaling pathway in osteoc...Continue Reading

References

Sep 1, 1996·Developmental and Comparative Immunology·E SolonR Gaugler
Oct 23, 1997·The Journal of Cell Biology·N R JorgensenT H Steinberg
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Mia M ThiDavid C Spray
Nov 7, 2003·American Journal of Physiology. Cell Physiology·Xiaoyong BaoLuis Reuss
Aug 12, 2004·FEBS Letters·Li BaoGerhard Dahl
Nov 5, 2005·The Journal of Biological Chemistry·Jiliang LiCharles H Turner
Feb 3, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sylvia O SuadicaniEliana Scemes
Apr 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J Ignacio AguirreTeresita Bellido
Jun 2, 2006·Molecular and Cellular Biology·Keqin ZhangLynda F Bonewald
Jun 28, 2006·IUBMB Life·Gerhard Dahl, Silviu Locovei
Aug 4, 2006·The Journal of Biological Chemistry·Gennady G YegutkinIvana Novak
Jan 24, 2007·FEBS Letters·Silviu LocoveiGerhard Dahl
Feb 16, 2007·Journal of Cellular Physiology·Damian C GenetosHenry J Donahue
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyong BaoGuillermo A Altenberg
Jul 27, 2007·American Journal of Physiology. Cell Physiology·Junjie WangGerhard Dahl
Jan 4, 2008·Pflügers Archiv : European journal of physiology·G Hawat, G Baroudi
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Keisuke SawadaYoshinori Moriyama
Apr 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gary Rudnick
Apr 22, 2008·Archives of Biochemistry and Biophysics·Roberto Civitelli
Jul 24, 2008·Cell Communication & Adhesion·Silvia PenuelaDale W Laird
Nov 22, 2008·The Journal of Pharmacology and Experimental Therapeutics·Weihong MaAnnmarie Surprenant
Jan 24, 2009·Progress in Neuro-psychopharmacology & Biological Psychiatry·Grzegorz R Juszczak, Artur H Swiergiel
Mar 5, 2009·Journal of Cellular Physiology·Isabel R OrrissTimothy R Arnett
Mar 21, 2009·Purinergic Signalling·Helle A Praetorius, Jens Leipziger
Jun 30, 2009·British Journal of Pharmacology·Diana L Donnelly-RobertsMichael F Jarvis
Jan 15, 2010·The Journal of Biological Chemistry·Andrew P FeranchakJ Gregory Fitz
Apr 13, 2010·The Journal of Biological Chemistry·Akihiro TokunagaShuji Kojima
May 6, 2010·The Biochemical Journal·Kristian A Haanes, Ivana Novak
Aug 18, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Christopher PriceLiyun Wang
May 4, 2011·Journal of Cellular Biochemistry·Saja A Al-DujailiLidan You
May 31, 2011·Frontiers in Bioscience (Scholar Edition)·Juan P ReyesS Jeffrey Dixon
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Alison GartlandJames A Gallagher
Mar 15, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David LuPaul A Insel
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Danielle WuDavid C Spray

❮ Previous
Next ❯

Citations

Sep 28, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Karan M ShahAlison Gartland
Feb 11, 2020·Annual Review of Physiology·Alexander G Robling, Lynda F Bonewald
Jul 18, 2020·Current Osteoporosis Reports·Wei CaoEngelbert A J M Schulten
Sep 6, 2019·Animals : an Open Access Journal From MDPI·Chris W Rogers, Keren E Dittmer
Apr 24, 2017·Cell and Tissue Research·Geoffrey Burnstock, Gillian E Knight
Aug 24, 2019·Bone Reports·Kylie A CorryJiliang Li
Jan 23, 2021·Biochemical Pharmacology·Ankita Agrawal, Niklas R Jørgensen
May 27, 2021·Journal of Orthopaedic Translation·Meng Chen Michelle LiWing Hoi Cheung
Jul 11, 2021·Bone·Yasaman Moharrer, Joel D Boerckel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.