Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth Factor vs Small Molecule-Based Approaches

Stem Cells International
Divya S VargheseSuraiya A Ansari

Abstract

Human embryonic stem cells (hESCs) are being utilized in diverse areas of studies such as development and disease modeling, cell replacement therapy, or drug toxicity testing because of their potential to be differentiated into any cell type in the body. The directed differentiation of hESCs into hepatocytes could provide an invaluable source of liver cells for various liver-based applications. Therefore, several protocols have been established in the past for hESC-hepatocyte differentiation based on the knowledge of signaling pathways and growth factors involved in different stages of embryonic hepatogenesis. Although successful derivation of hepatocytes has been achieved through these protocols, the efficiency is not always ideal. Herein, we have tested several combinations of published protocols, for example, growth factor vs. small molecule and different time durations of treatment for definitive endoderm (DE) induction and further hepatocyte differentiation to develop an efficient DE induction and hepatocyte differentiation in a highly reproducible manner based on the stage-specific marker expression and functional analysis.

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Citations

Jun 23, 2019·International Journal of Molecular Sciences·Wachira PantaRangsun Parnpai
Dec 6, 2019·Frontiers in Medicine·James L Corbett, Stephen A Duncan
Sep 2, 2020·International Journal of Molecular Sciences·Cristina OlgasiAntonia Follenzi
Oct 13, 2020·Journal of Tissue Engineering and Regenerative Medicine·Nima ZakeriMehran Solati-Hashjin
Nov 28, 2020·Colloids and Surfaces. B, Biointerfaces·M R PoornaUllas Mony

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Methods Mentioned

BETA
PCR
Assay

Software Mentioned

EVOS XL
GraphPad

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