Fine-tuning scaffolds for tissue regeneration: effects of formic acid processing on tissue reaction to silk fibroin

Journal of Tissue Engineering and Regenerative Medicine
Shahram M GhanaatiC James Kirkpatrick

Abstract

Formic acid (FA) plays a key role in the preparation of silk fibroin (SF) scaffolds from cocoons of Bombyx mori and is used for fibre distribution. In this study, we used a subcutaneous implantation model in Wistar rats to examine SF scaffolds prepared by treating the degummed cocoon with FA for either 30 or 60 min. The tissue reaction and inflammatory response to SF was assessed by qualitative histology at intervals from 3 to 180 days. Additionally, dynamic biomaterial-induced vascularization and biomaterial degradation were quantified using a technique for analysing an image of the entire implanted biomaterial. Varying the FA treatment time led to different scaffold morphologies and resulted in two distinct peri-implant tissue reactions. The 30 min-treated scaffold was integrated into the surrounding tissue beginning at day 3 after implantation and vascularization increased 10-fold from 15 to 180 days, while the scaffold was continuously degraded throughout the first 90 days. In contrast, the 60 min-treated SF scaffold appeared as bulk for the first 90 days after implantation, after which a rapid degradation and vascularization process began. After 180 days, the tissue response was similar for both scaffolds, with eventual fo...Continue Reading

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Citations

Dec 17, 2010·European Journal of Medical Research·S GhanaatiB Willershausen
Oct 1, 2015·Journal of Biomedical Materials Research. Part a·Mike BarbeckShahram Ghanaati
Apr 18, 2013·Journal of Tissue Engineering and Regenerative Medicine·Valentina Di FeliceGiovanni Zummo
Nov 6, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Noboru KuboyamaNorihiro Nishiyama
May 24, 2011·Journal of Tissue Engineering and Regenerative Medicine·Shahram GhanaatiC James Kirkpatrick
Feb 11, 2011·Journal of Biomedical Materials Research. Part a·Rangam RajkhowaXungai Wang
Sep 14, 2012·Journal of Tissue Engineering and Regenerative Medicine·Antonella MottaClaudio Migliaresi
Sep 1, 2015·Biomaterials·Amy E ThurberDavid L Kaplan
Mar 31, 2015·Biomaterials·Cristina SerranoAránzazu del Campo
Feb 18, 2014·Acta Biomaterialia·Andreas H TeuschlHeinz Redl
Jun 20, 2014·The Journal of Oral Implantology·Shahram GhanaatiJoseph Choukroun
Sep 21, 2016·Journal of Functional Biomaterials·Michael FlorenAntonella Motta
Dec 7, 2017·Clinical Oral Investigations·Po-Ju Chia-LaiShahram Ghanaati
Nov 22, 2017·Journal of Tissue Engineering and Regenerative Medicine·Hsin-Yu LoShoei-Shen Wang
Oct 27, 2017·Journal of Materials Science. Materials in Medicine·Simon WendShahram Ghanaati
May 9, 2016·ACS Biomaterials Science & Engineering·Emanuela CalloneAntonella Motta

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