PMID: 8946348Dec 1, 1996Paper

Finger printing of Mycobacterium tuberculosis in patients with intracranial tuberculomas by using in vivo, ex vivo, and in vitro magnetic resonance spectroscopy

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
R K GuptaA P Bhaduri

Abstract

In vivo, ex vivo, and in vitro proton magnetic resonance spectroscopy was performed in 12 patients with intracranial tuberculomas with an aim of detecting the biochemical constituents of Mycobacterium tuberculosis in a granuloma. One dimensional (1D) single pulse and spin-echo sequences and 2D correlative spectroscopy were used for the ex vivo study to confirm the resonances seen on in vivo study. Spectroscopic studies of the perchloric acid and lipid extract of granuloma and M. tuberculosis were performed to look for similarity of resonance. In vivo study showed the presence of lipids at 0.9, 1.3, 2.0, 2.8 ppm, and phosphoserine at 3.7 ppm. All these resonances were confirmed on ex vivo study. In addition, distinct resonances of serine and phenolic lipids were seen on ex vivo and in vitro study of tuberculous granuloma, which have not been observed in other intracranial tumors. Lipid extract of granuloma and M. tuberculosis showed phenolic lipids at 7.1 and 7.4 ppm, a constituent of the cell wall of the bacteria in a tuberculoma. It appears that it may be possible to finger print the biochemicals of the cell wall of M. tuberculosis in a tuberculous granuloma and thus may help in detection and diagnosis of such lesions.

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Citations

May 26, 2005·European Journal of Radiology·Carlos Morgado, Nuno Ruivo
Jun 19, 2013·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Arthur R DeLanceAndrew T Parsa
Jan 1, 2005·Topics in Magnetic Resonance Imaging : TMRI·Meng Law
Nov 3, 2009·The Indian Journal of Radiology & Imaging·Richa TrivediRakesh K Gupta
Feb 7, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ronan J MurrayTania C Sorrell
Jun 25, 2014·Journal of Neuroradiology. Journal De Neuroradiologie·Carmine Franco MuccioCosma Andreula
Jun 3, 2014·Topics in Magnetic Resonance Imaging : TMRI·Carlos TorresRakesh K Gupta
Oct 3, 2014·World Journal of Radiology·Krithika RangarajanArun Kumar Gupta
Jun 10, 2006·NMR in Biomedicine·Peter B KingsleyRona Woldenberg
Jun 1, 2007·Journal of Computer Assisted Tomography·Rakesh K GuptaRam Kishore S Rathore
Jul 31, 2008·Journal of Computer Assisted Tomography·Chandan Jyoti Das, Kunjahari Medhi
Oct 14, 2005·Magnetic Resonance in Medical Sciences : MRMS : an Official Journal of Japan Society of Magnetic Resonance in Medicine·Keiko Imamura
Jul 22, 1999·NMR in Biomedicine·R JayasundarA K Banerji
Jan 31, 2019·Indian Journal of Pediatrics·Anuradha DawaniManisha Jana
Oct 29, 2011·Neuroimaging Clinics of North America·Rakesh K Gupta, Sunil Kumar

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