Fingerprint analysis of Dioscorea nipponica by high-performance liquid chromatography with evaporative light scattering detection

Analytica Chimica Acta
Chun-Zhao LiuQiong Yan

Abstract

High-performance liquid chromatographic method (HPLC) with evaporative light scattering detection (ELSD) coupled with microwave-assisted extraction (MAE) as an efficient sample preparation technique has been developed for fingerprint analysis of Dioscorea nipponica. The samples were separated with an Agilent C8 column using water (A) and acetonitrile (B) under gradient conditions (0-10 min, linear gradient 20-40% B; 10-12 min, linear gradient 40-42% B; 12-25 min, isocratic 42% B) as the mobile phase at a flow rate of 1 mL min(-1) within 22 min. The ELSD conditions were optimized at nebulizer-gas flow rate 2.7 L min(-1) and drift tube temperature 90 degrees C. Precision experiments showed relative standard deviation (R.S.D.) of peak area and retention time were better than 2.5%; inter-day and intra-day variabilities showed that R.S.D. was ranged from 0.78% to 4.74%. Limit of detection was less than 50 microg mL(-1) and limit of quantification was less than 80 microg mL(-1). Accuracy validation showed that average recovery was between 97.39% and 104.07%. The method was validated to achieve the satisfactory precision and recovery. Relative retention time and relative peak area were used to identify the common peaks for fingerprint...Continue Reading

References

May 13, 2004·Journal of Pharmaceutical and Biomedical Analysis·Jun ChenQi-Zhi Zhang
Sep 8, 2004·Journal of Pharmaceutical and Biomedical Analysis·Nikolaos C Megoulas, Michael A Koupparis
Jan 28, 2005·The American Journal of Chinese Medicine·Yu Nan ZhaoLijun Du
Feb 11, 2005·Journal of Chromatography. a·Devanand P Fulzele, Ramesh K Satdive
Feb 3, 2006·Journal of Chromatography. a·W Oleszek, Z Bialy
Mar 28, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hua-Ying Zhou, Chun-Zhao Liu

❮ Previous
Next ❯

Citations

Jan 3, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ming-Hsien ChienShun-Fa Yang
Sep 21, 2007·Journal of Separation Science·Loretta Pobłocka-OlechTomasz Baczek
Sep 1, 2013·Journal of Pharmacopuncture·Min-Jung KimKwon-Eui Hong
May 26, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Bashir Mohammed AbubakarAlina Wagiran

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.