Fingerprint of long non-coding RNA regulated by cyclic mechanical stretch in human aortic smooth muscle cells: implications for hypertension

Molecular and Cellular Biochemistry
Laura-Eve MantellaSubodh Verma

Abstract

Emerging evidence suggests that long non-coding RNAs (lncRNAs) represent a cellular hub coordinating various cellular processes that are critical in health and disease. Mechanical stress triggers changes in vascular smooth muscle cells (VSMCs) that in turn contribute to pathophysiological changes within the vasculature. We sought to evaluate the role that lncRNAs play in mechanical stretch-induced alterations of human aortic smooth muscle cells (HASMCs). RNA (lncRNA and mRNA) samples isolated from HASMCs that had been subjected to 10 or 20% elongation (1 Hz) for 24 h were profiled with the Arraystar Human LncRNA Microarray V3.0. LncRNA expression was quantified in parallel via qRT-PCR. Of the 30,586 human lncRNAs screened, 580 were differentially expressed (DE, P < 0.05) in stretched HASMCs. Amongst the 26,109 protein-coding transcripts evaluated, 25 of those DE were associated with 25 of the aforementioned DE lncRNAs (P < 0.05). Subsequent Kyoto Encyclopedia of Genes and Genomes analysis revealed that the DE mRNAs were largely associated with the tumor necrosis factor signaling pathway and inflammation. Gene Ontology analysis indicated that the DE mRNAs were associated with cell differentiation, stress response, and response t...Continue Reading

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Citations

Sep 29, 2020·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Ji-Ru Zhang, Hai-Jian Sun
Feb 6, 2020·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xiaoying Jiang, Qilan Ning
Mar 7, 2019·Frontiers in Cardiovascular Medicine·Adam W TurnerClint L Miller

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Methods Mentioned

BETA
PCR
Feature Extraction
zymography
dissection

Software Mentioned

Agilent Feature Extraction
bioconductor
topGO
GeneSpring GX
Arraystar
GraphPad Prism
ImageJ

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