Finite-element analysis of geometrical factors in micro-indentation of pollen tubes

Biomechanics and Modeling in Mechanobiology
J-E BolducAnja Geitmann

Abstract

Micro-indentation is a new experimental approach to assess physical cellular properties. Here we attempt to quantify the contribution of geometrical parameters to a cylindrical plant cell's resistance to lateral deformation. This information is important to correctly interpret data obtained from experiments using the device, such as the local cellular stiffness in pollen tubes. We built a simple finite-element model of the micro-indentation interacting partners - micro-indenter, cell (pollen tube), and underlying substratum, that allowed us to manipulate geometric variables, such as geometry of the cell, cell radius, thickness of the cell wall and radius of the indenting stylus. Performing indentation experiments on this theoretical model demonstrates that all four parameters influence stiffness measurement and can therefore not be neglected in the interpretation of micro-indentation data.

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Citations

Feb 1, 2012·Journal of Experimental Botany·Céline M HayotJoseph A Turner
Feb 23, 2012·Plant Physiology·Anne-Lise Routier-KierzkowskaRichard S Smith
Aug 12, 2010·The Plant Cell·Pierre FayantAnja Geitmann
Feb 23, 2010·BMC Plant Biology·Shannon F StewmanAaron R Dinner
Jul 23, 2013·Journal of Experimental Botany·Pascale MilaniArezki Boudaoud
Sep 26, 2013·Journal of Experimental Botany·Ingo Burgert, Tobias Keplinger
Feb 22, 2011·Annual Review of Plant Biology·Vincent MirabetOlivier Hamant
Jan 10, 2012·Annual Review of Plant Biology·Sebastian WolfHerman Höfte
Mar 3, 2010·Annual Review of Plant Biology·Vijay ChickarmaneElliot M Meyerowitz
Jan 1, 2015·Plants·Hannes VoglerUeli Grossniklaus
Jul 7, 2011·Seminars in Cell & Developmental Biology·Yuan Qin, Zhenbiao Yang
Oct 26, 2010·Current Opinion in Plant Biology·Anja Geitmann
Oct 31, 2012·The Plant Journal : for Cell and Molecular Biology·Hannes VoglerUeli Grossniklaus
Oct 6, 2012·The Plant Journal : for Cell and Molecular Biology·Elham ForouzeshJoseph A Turner
Apr 16, 2015·Journal of Experimental Botany·Alain WeberRichard S Smith
Dec 12, 2012·Current Opinion in Plant Biology·Anne-Lise Routier-Kierzkowska, Richard S Smith
Jun 24, 2016·American Journal of Botany·Anna F EdlundDaphne Preuss
Sep 1, 2012·American Journal of Botany·Otger CampàsL Mahadevan
Jul 11, 2019·Journal of Experimental Botany·Wojtek PałubickiAgata Burian
Jul 14, 2019·Journal of Experimental Botany·Amir J Bidhendi, Anja Geitmann

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