Jan 15, 2020

Finite Element Modelling of Single Cell Based on Atomic Force Microscope Indentation Method

Computational and Mathematical Methods in Medicine
Lili WangWeiyi Chen

Abstract

The stiffness of cells, especially cancer cells, is a key mechanical property that is closely associated with their biomechanical functions, such as the mechanotransduction and the metastasis mechanisms of cancer cells. In light of the low survival rate of single cells and measurement uncertainty, the finite element method (FEM) was used to quantify the deformations and predict the stiffness of single cells. To study the effect of the cell components on overall stiffness, two new FEM models were proposed based on the atomic force microscopy (AFM) indentation method. The geometric sizes of the FEM models were determined by AFM topography images, and the validity of the FEM models was verified by comparison with experimental data. The effect of the intermediate filaments (IFs) and material properties of the cellular continuum components on the overall stiffness were investigated. The experimental results showed that the stiffness of cancer cells has apparent positional differences. The FEM simulation results show that IFs contribute only slightly to the overall stiffness within 10% strain, although they can transfer forces directly from the membrane to the nucleus. The cytoskeleton (CSK) is the major mechanical component of a cel...Continue Reading

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Mentioned in this Paper

Cellular Structures
Study
Biomechanics
Arterial Stiffness
Connective Tissue Cells
Simulation
Membrane
Stiffness
Cell Nucleus
Mechanotransduction, Cellular

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