FIP-fve stimulates interferon-gamma production via modulation of calcium release and PKC-alpha activation

Journal of Agricultural and Food Chemistry
Chu-Chyn OuMeei-Yn Lin

Abstract

Fungal immunomodulatory protein, FIP-fve, has been isolated from Flammulina velutipes, and its immunomodulatory effects are believed to be associated with the enhanced activation of IFN-gamma-releasing Th1 cells. However, the mechanisms of FIP-fve-mediated signal transduction in the regulation of interferon-gamma (IFN-gamma) gene expression in human peripheral blood mononuclear cells (PBMCs) are still poorly understood. Using fluo-3 AM, we found that FIP-fve induces a rapid elevation in calcium concentration. ELISA, RT-PCR and Western blot assays demonstrated significant increases in the production and mRNA expression of IFN-gamma and protein kinase C-alpha (PKC-alpha) activation in activated PBMCs, which were abolished by EGTA, nifedipine and GO6976. In conclusion, Ca2+ release and PKC-alpha activation are required for IFN-gamma production induced by FIP-fve in PBMCs.

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Citations

Apr 25, 2012·Journal of Agricultural and Food Chemistry·Yu-Fan LiuJiunn-Liang Ko
Jun 14, 2018·Current Protein & Peptide Science·Rong ZhouFang Liu
Jun 10, 2011·Critical Reviews in Biotechnology·Qi-Zhang LiXuan-Wei Zhou
Oct 6, 2020·Frontiers in Nutrition·Yusi LiuHarry J Wichers
May 25, 2011·Biotechnology Advances·Xiaofei XuXuewu Zhang

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