PMID: 9163352Apr 15, 1997Paper

Fip-vvo, a new fungal immunomodulatory protein isolated from Volvariella volvacea

The Biochemical Journal
H C HsuJ Y Lin

Abstract

A new fungal immunomodulatory protein (Fip) has been purified from the edible mushroom, Volvariella volvacea, and designated Fip-vvo. Analysis of the purified protein by SDS/PAGE followed by Coomassie Blue staining demonstrated that Fip-vvo is a single polypeptide with an apparent molecular mass of 15 kDa. Periodic acid/Schiff staining showed that this single polypeptide lacks carbohydrates. Using an in vitro bioassay measuring blast-formation stimulatory activity, Fip-vvo was shown to stimulate the maximum proliferation of human peripheral blood lymphocytes at a concentration of 5 microg/ml. Fip-vvo was capable of agglutinating rat red blood cells. Neither haemagglutination nor mitogenic activities were inhibited by mono- or dimeric sugars. In vivo, repeat administration of Fip-vvo greatly reduced the production of BSA-induced Arthus reaction in mice, whereas little effect was observed on the prevention of systemic anaphylaxis reactions. The selectively enhanced transcriptional expression of interleukin (IL)-2, IL-4, interferon-gamma, tumour necrosis factor-alpha, lymphotoxin and IL-2 receptor by Fip-vvo was also demonstrated by reverse transcriptase-PCR. This finding suggests that Fip-vvo exerts its immunomodulatory effects v...Continue Reading

Citations

May 7, 2002·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Linda S M OoiM C Fung
Dec 29, 2010·Biochemistry Research International·Nagaraja N NagreShashikala R Inamdar
Jul 21, 2015·Food & Function·Suhas TalmaleMandakini Patil
Jun 14, 2018·Current Protein & Peptide Science·Rong ZhouFang Liu
Apr 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nakarin SuwannarachSaisamorn Lumyong
Oct 6, 2020·Frontiers in Nutrition·Yusi LiuHarry J Wichers
Jan 24, 2009·The Journal of General and Applied Microbiology·Qin XueMingwen Zhao
Dec 12, 2012·Critical Reviews in Microbiology·Ram Sarup Singh, Amandeep K Walia
Apr 19, 2011·Cancer Immunology, Immunotherapy : CII·Chi-Chen LinChing-Liang Chu
May 21, 2009·Analytical and Bioanalytical Chemistry·Harry Wichers
Feb 26, 2008·Experimental Biology and Medicine·Andrea T BorchersM Eric Gershwin
Oct 20, 2017·Applied Microbiology and Biotechnology·Lijuan HouZhengpeng Li
Jan 29, 2010·Critical Reviews in Biotechnology·Ram Sarup SinghHemant Preet Kaur
May 19, 2011·Applied Biochemistry and Biotechnology·Ram Sarup SinghAshok Kumar Tiwary
May 10, 2013·Immunopharmacology and Immunotoxicology·Hui ZhouChongyang Liang
Jun 20, 2020·Applied Microbiology and Biotechnology·Vala RezvaniLeila Farahmand
Nov 22, 2019·International Journal of Biological Macromolecules·Ram Sarup SinghJohn F Kennedy
Jan 12, 2021·International Journal of Biological Macromolecules·Ashwin B ButleTrimurti L Lambat

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