Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation

Chembiochem : a European Journal of Chemical Biology
Hugo FragaJoaquim C G Esteves da Silva

Abstract

Firefly luciferase catalyzes the synthesis of H2O2 from the same substrates as the bioluminescence reaction: ATP and luciferin (D-LH2). About 80% of the enzyme-bound intermediate D-luciferyl adenylate (D-LH2-AMP) is oxidized into oxyluciferin, and a photon is emitted during this reaction. The enzyme pathway responsible for the generation of H2O2 is a side reaction in which D-LH2-AMP is oxidized into dehydroluciferyl adenylate (L-AMP). Like the bioluminescence reaction, the luciferase-catalyzed synthesis of H2O2 and L-AMP is a stereospecific process, involving only the natural D enantiomer. However, the intramolecular electron transfer postulated as essential to the light emission process is not involved in this side reaction.

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J A KooG B Schuster
May 1, 1989·Photochemistry and Photobiology·B R BranchiniE J Lajiness
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G A KellerS Subramani
Jun 1, 1968·Angewandte Chemie·T Goto, Y Kishi
Jan 1, 1995·Critical Reviews in Toxicology·A R GoeptarN P Vermeulen
Jul 1, 1996·The Biochemical Journal·N Lembert
Aug 1, 1997·The Journal of Biological Chemistry·B R BranchiniM H Murtiashaw
Oct 24, 1997·Photochemistry and Photobiology·C H ContagD A Benaron
Mar 7, 2002·Journal of the American Chemical Society·Bruce R BranchiniJustin G Stroh
Mar 27, 2002·Methods in Enzymology·Kevin R Messner, James A Imlay
Apr 10, 2002·Circulation·Joseph C WuSanjiv S Gambhir
Jan 18, 2003·Cellular and Molecular Life Sciences : CMLS·V R Viviani
Mar 8, 2003·Methods in Enzymology·J Woodland Hastings, Carl Hirschie Johnson
Oct 1, 1956·Archives of Biochemistry and Biophysics·A GREEN, W D MCELROY
Mar 1, 1958·Biochimica Et Biophysica Acta·R L AIRTHW D McELROY
Aug 1, 1961·Proceedings of the National Academy of Sciences of the United States of America·H H SELIGERG F FIELD
Dec 26, 2003·Chembiochem : a European Journal of Chemical Biology·Hugo FragaRui Fontes
Apr 27, 2005·Biochemical and Biophysical Research Communications·Mitsuhiro NakamuraHaruki Niwa

❮ Previous
Next ❯

Citations

Apr 26, 2008·Analytical and Bioanalytical Chemistry·Simone M Marques, Joaquim C G Esteves da Silva
Dec 19, 2012·The Journal of Physical Chemistry. a·Luís Pinto da SilvaJoaquim C G Esteves da Silva
Feb 12, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hugo Fraga
Aug 30, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·César Ribeiro, Joaquim C G Esteves da Silva
May 29, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Luís Pinto da Silva, Joaquim C G Esteves da Silva
Jan 21, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Huateng ZhangMinyong Li
Jan 11, 2011·Journal of Photochemistry and Photobiology. B, Biology·Simone M MarquesJoaquim C G Esteves da Silva
Jun 17, 2008·International Journal of Biological Macromolecules·Maryam MehrabiSirous Ghobadi
May 2, 2007·Journal of Photochemistry and Photobiology. B, Biology·V N Petushkov, N S Rodionova
Oct 25, 2008·IUBMB Life·Simone M Marques, Joaquim C G Esteves da Silva
Jul 12, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Luís Pinto da SilvaJoaquim C G Esteves da Silva
Feb 23, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Luís Pinto da Silva, Joaquim C G Esteves da Silva
Nov 26, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Chun-Gang MinJi-Kang Feng
Jul 23, 2016·Frontiers in Oncology·Tingting XuSteven Ripp
May 19, 2016·The FEBS Journal·Azadeh Lohrasbi-NejadSaman Hosseinkhani
Feb 1, 2018·Chembiochem : a European Journal of Chemical Biology·Brendan S ZhangJennifer A Prescher
Dec 12, 2017·ChemistryOpen·Michael C PirrungJiayu Liao
Apr 6, 2017·Organic & Biomolecular Chemistry·Carla T SalatinoLuiz F M L Ciscato
Jan 3, 2015·Physical Review Letters·Yueh-Lin TsaiY Hwu
Nov 27, 2020·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M C CarvalhoV R Viviani
Mar 19, 2021·Chemical Society Reviews·Aisha J Syed, James C Anderson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
César Ribeiro, Joaquim C G Esteves da Silva
© 2022 Meta ULC. All rights reserved