First birth following assisted sperm fusion insemination using sperm bound to zona pellucida.

Journal of Assisted Reproduction and Genetics
Shota HatakeyamaAtsushi Yanaihara

Abstract

To report a live birth after transfer of a vitrified-warmed blastocyst produced by assisted sperm fusion insemination (ASFI). Oocyte retrieval and in vitro fertilization (IVF) were performed on a 37-year-old woman. Six hours after IVF, an oocyte exhibited a single polar body and so was defined as an unfertilized oocyte. A motile sperm was collected from the zona pellucida of the unfertilized oocyte by an injection needle. The motile sperm was pressed onto the membrane of the unfertilized oocyte. Two oocytes were matured and subjected to IVF. One of the 2 oocytes exhibited only one polar body and was defined as an unfertilized oocyte at 6 h after IVF; this oocyte then was subjected to ASFI. Two pronuclei were observed on the next day and cultured to the blastocyst stage. This embryo achieved blastocyst status and was vitrified on day 5. The resulting vitrified-warmed blastocyst was transferred, resulting in pregnancy and subsequent delivery of a healthy boy. This report describes the first case of a successful birth following transfer of a vitrified-warmed blastocyst produced by ASFI.

References

Dec 1, 1993·Human Reproduction·Z P NagyA C Van Steirteghem
Oct 24, 2000·Biology of the Cell·C PatratP Jouannet
Jul 15, 2003·Current Opinion in Obstetrics & Gynecology·Neal G Mahutte, Aydin Arici
Jun 5, 2008·Journal of Cell Science·Claire V HarperPeter M Johnson
Jan 22, 2014·Fertility and Sterility·Ronit Beck-FruchterEliezer Shalev
May 16, 2014·Journal of Assisted Reproduction and Genetics·Linjun ChenHaixiang Sun
Feb 17, 2018·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Yuxia HeJianqiao Liu

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