First bone formation and the dissection of an osteogenic lineage in the embryonic chick tibia is revealed by monoclonal antibodies against osteoblasts.

Bone
S P Bruder, A I Caplan

Abstract

Detailed studies of the origin and differentiation of osteogenic cells can be facilitated by cell-specific markers. To this end, we immunized mice with a heterogeneous population of chick embryonic bone cells and subsequently generated and selected for monoclonal antibodies against cell surface determinants. Supernatants from growing hybridoma colonies were screened immunohistochemically against frozen sections of embryonic stage 35 (day 9.5) chick tibiae. Three cell lines, SB-1, SB-2, and SB-3, which each secrete a different antibody against osteogenic cells, have been successfully cloned, stabilized, and immortalized. Antibody SB-1 reacts with a family of cells in embryonic bone, liver, kidney, and intestine, which are identically stained by the histochemical stain for alkaline phosphatase. The SB-2 antigen is present only on osteoblasts, while the SB-3 antigen is expressed on the surface of osteoblasts, ependymal cells and ventricular myoblasts. Studies on the developmental progression of osteoblasts in the embryonic tibia indicate that the determinants recognized by SB-1, SB-2, and SB-3 are temporally coupled to the appearance of the pre-osteoblast marker alkaline phosphatase. Detailed morphologic analyses reveal that SB-1 ...Continue Reading

References

Dec 1, 1979·Journal of Morphology·P Scott-Savage, B K Hall
Dec 22, 1975·Calcified Tissue Research·Y ShimomuraF Suzuki
Dec 1, 1987·Developmental Biology·Z Kaprielian, D M Fambrough
Dec 17, 1982·Journal of Immunological Methods·G D JohnsonE J Holborow
Aug 1, 1981·Developmental Biology·P Osdoby, A I Caplan
Jan 1, 1980·Acta Anatomica·P Scott-Savage, B K Hall
Oct 1, 1963·The Journal of Cell Biology·M OWEN, S MACPHERSON
Jan 1, 1934·The Biochemical Journal·H B Fell, R Robison
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Feb 1, 1996·Microscopy Research and Technique·J E Aubin, K Turksen
Nov 11, 1999·Journal of Biomedical Materials Research·H Ohgushi, A I Caplan
Feb 1, 1997·Journal of Cellular Biochemistry·N JaiswalS P Bruder
Jul 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K WatanabeA I Caplan
Sep 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G L WongD E Swartzendruber
Jan 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C GrayT R Arnett
Nov 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K TingI Nishimura
Dec 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B D Boyan, Z Schwartz
Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E MathieuJ Merregaert
Nov 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S WalshM Gowen
Oct 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D BenayahuS Wientroub
Nov 1, 1994·Journal of Cellular Biochemistry·S P BruderA I Caplan
Sep 1, 1993·In Vitro Cellular & Developmental Biology. Animal·J Vilamitjana-AmedeeM F Harmand
Aug 1, 1995·Bone·J E AubinA K Gupta
Feb 8, 2011·Journal of Biomaterials Science. Polymer Edition·Kaige LüFuqiang Zhang
Jan 8, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C D TomaL C Gerstenfeld
Feb 23, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·X D ChenM C Horowitz
Jul 22, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yuichi NambaKeitaro Isokawa
May 28, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Arnold I Caplan, Diego Correa
Jan 1, 1992·Connective Tissue Research·C J JoynerJ T Triffitt
Aug 13, 2010·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Y YamazakiK Isokawa
Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Jun 26, 2020·Frontiers in Molecular Neuroscience·Yunus H OzekinEmily A Bates
Jan 1, 1992·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J B Lian, G S Stein

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