First data on in vitro fertilization and blastocyst formation after intraovarian injection of calcium gluconate-activated autologous platelet rich plasma

Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology
E Scott SillsGianpiero D Palermo

Abstract

Platelets modulate clinically relevant yet incompletely understood tissue regeneration processes, and platelet rich plasma (PRP) has been previously used with some success in various non-reproductive medical contexts. Here, we extended PRP application to ovarian tissue with a view to document impact on ovarian reserve among women attending for infertility treatment. PRP was freshly isolated from patients (n= 4) with diminished ovarian reserve as determined by at least one prior IVF cycle canceled for poor follicular recruitment response or estimated by serum AMH and/or FSH, no menses for ≥1 year. Immediately following substrate isolation and activation with calcium gluconate, approximately 5 mL of autologous PRP was injected into each ovary under direct transvaginal sonogram guidance. For each study subject, AMH, FSH, and serum estradiol data were recorded at two-week intervals post-PRP and compared to baseline (pre-PRP) values. In this pilot group, mean (±SD) patient age was 42 ± 4 years with infertility duration reported as 60 ± 25 months. Following this protocol of intraovarian PRP administration, increases in serum AMH (p = .17), decreases in FSH (p < .01), or both, were observed in all cases, sufficient to permit retrieval...Continue Reading

References

Jan 1, 1997·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·P F MannaioniE Masini
Dec 24, 2003·Thrombosis and Haemostasis·Eduardo AnituaAlan T Nurden
Mar 12, 2004·Nature·Joshua JohnsonJonathan L Tilly
May 16, 2008·Nature·Geoffrey C GurtnerMichael T Longaker
Apr 24, 2010·Seminars in Thrombosis and Hemostasis·Konstantinos StellosHarald F Langer
Feb 24, 2011·AJR. American Journal of Roentgenology·Kenneth S LeeCamilo G Borrero
Apr 12, 2011·Thrombosis and Haemostasis·Alan T Nurden
Jun 30, 2011·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Antonin Bukovsky
Jan 11, 2012·Current Pharmaceutical Design·Irma Virant-KlunThomas Skutella
Mar 8, 2012·Nature Medicine·Evelyn E Telfer, David F Albertini
May 2, 2012·Obstetrics and Gynecology International·Deepa BhartiyaSeema Parte
Jul 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hua ZhangKui Liu
May 25, 2013·Ginekologia polska·Monika Szafarowska, Małgorzata Jerzak
Jun 12, 2013·Cell Metabolism·Jonathan L Tilly, David A Sinclair
Oct 2, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Deepa Bhartiya, Jarnail Singh
Feb 28, 2015·Journal of Cutaneous and Aesthetic Surgery·Maria-Angeliki GkiniKonstantinos Kouskoukis
Sep 10, 2015·Nature Reviews. Molecular Cell Biology·Annie Ng, Nick Barker
Jul 26, 2017·Fertility and Sterility·Francisca Martinez, UNKNOWN International Society for Fertility Preservation–ESHRE–ASRM Expert Working Group

❮ Previous
Next ❯

Citations

Jul 21, 2020·Cell Transplantation·Konstantinos SfakianoudisMara Simopoulou
Dec 24, 2018·Journal of Clinical Medicine·Konstantinos SfakianoudisKonstantinos Pantos
Sep 10, 2020·Frontiers in Veterinary Science·Fausto CremonesiAnna Lange-Consiglio
Sep 17, 2020·Diagnostics·Polina GiannelouKonstantinos Sfakianoudis
Mar 17, 2021·Journal of Assisted Reproduction and Genetics·Fady I ShararaGaby N Moawad
Mar 16, 2021·Frontiers in Endocrinology·Alba M PolonioSonia Herraiz
May 9, 2021·Human Reproduction·Lloyd AtkinsonRoger G Sturmey
Jul 20, 2021·Advanced Drug Delivery Reviews·José das NevesBruno Sarmento

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.