First description of a laccase-like enzyme in soil algae

Archives of Microbiology
Benjamin OttoWerner Reisser

Abstract

Laccases (EC 1.10.3.2) are versatile multi-copper oxidases so far found in higher plants, fungi, insects, prokaryotes and lichens. In the present study, the production of an extracellular laccase-like enzyme by the coccoid green soil alga Tetracystis aeria was investigated and the enzyme was partly characterized, thereby providing the first description of a laccase-like enzyme in soil algae. Enzyme production in algae cultures was considerably increased by addition of the fungal laccase inducer copper sulphate. Maximal enzyme production was observed during the stationary growth phase. Peroxidase or tyrosinase activity was not detected. The native enzyme exhibits an apparent molecular mass of about 212 kDa as observed with size exclusion chromatography and about 210-260 kDa as estimated by zymograms. The enzyme efficiently oxidizes 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,6-dimethoxyphenol (2,6-DMP), syringaldazine (SGZ) and the anthraquinone dye Acid Blue 62, while guaiacol and Remazol Brilliant Blue R are only poorly oxidized. The apparent kinetic parameters obtained for ABTS, 2,6-DMP and SGZ oxidation are within the range reported for fungal laccases. Oxidation of the phenolic substrate 2,6-DMP display...Continue Reading

References

Mar 4, 2000·Applied and Environmental Microbiology·G PalmieriG Sannia
Jul 18, 2002·Journal of Inorganic Biochemistry·Kazutomo NittaTakeshi Sakurai
Jul 20, 2002·Phytochemistry·Alfred M Mayer, Richard C Staples
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Harald Claus
Aug 5, 2004·Applied Microbiology and Biotechnology·H J Ruijssenaars, S Hartmans
Feb 14, 2006·FEMS Microbiology Reviews·Petr Baldrian
Mar 15, 2006·Applied and Environmental Microbiology·N DasM Mukherjee
Apr 1, 2006·Trends in Biotechnology·Sergio Riva
Jul 1, 1992·Plant Physiology·R SterjiadesK E Eriksson
Aug 1, 2007·Photosynthesis Research·George C PapageorgiouKostas Stamatakis
Aug 8, 2007·FEMS Microbiology Letters·Alexander V LisovAlexey A Leontievsky
Feb 6, 2008·Journal of Neuro-oncology·G MinnitiR Maurizi Enrici
Oct 22, 2009·Cellular and Molecular Life Sciences : CMLS·Paola GiardinaGiovanni Sannia

❮ Previous
Next ❯

Citations

May 21, 2013·Enzyme and Microbial Technology·Joseph O RichPennapa Manitchotpisit
Dec 4, 2012·Enzyme and Microbial Technology·Fabiola MedinaRafael Vazquez-Duhalt
Dec 7, 2011·Enzyme and Microbial Technology·Pasquale ChiaieseEdgardo Filippone
Aug 6, 2016·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Mark D Cannatelli, Arthur J Ragauskas
Dec 30, 2020·Bioresource Technology·Tatyana N ShchemelininaDmitry A Shushkov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.