First description of SHV-148 mediated extended-spectrum cephalosporin resistance among clinical isolates of Escherichia coli from India

Indian Journal of Medical Microbiology
Anand Prakash MauryaA Bhattacharjee

Abstract

The present study was aimed to investigate the genetic context, association with IS26 and horizontal transmission of SHV-148 among Escherichia coli in Tertiary Referral Hospital of India. Phenotypic characterisation of extended-spectrum beta-lactamases (ESBLs) was carried out as per CLSI criteria. Molecular characterisation of blaSHVand integron was carried out by polymerase chain reaction (PCR) assay and confirmed by sequencing. Linkage of IS26 with blaSHV-148was achieved by PCR. Purified products were cloned on pGEM-T vector and sequenced. Strain typing was performed by pulsed field gel electrophoresis with Xba I digestion. Transferability experiment and antimicrobial susceptibility was performed. A total of 33 isolates showed the presence of SHV-148 variant by sequencing and all were Class 1 integron borne. PCR and sequencing results suggested that all blaSHV-148 showed linkage with IS26 and were present in the upstream portion of the gene cassette and were also horizontally transferable through F type of Inc group. Susceptibility results suggest that tigecycline was most effective. The present study reports for the first time of SHV-148 mediated extended spectrum cephalosporin resistance from India. Association of their res...Continue Reading

References

Jan 4, 2001·Journal of Clinical Microbiology·J G KoelemanP H Savelkoul
May 14, 2005·The Journal of Antimicrobial Chemotherapy·Seungok LeeMoon Won Kang
Jun 7, 2005·Journal of Microbiological Methods·Alessandra CarattoliE John Threlfall
Nov 18, 2005·The Journal of Antimicrobial Chemotherapy·C EckertG Arlet
Jan 5, 2008·The Journal of Antimicrobial Chemotherapy·Amitabha BhattacharjeeShampa Anupurba
Feb 23, 2008·The Lancet Infectious Diseases·Johann D D Pitout, Kevin B Laupland
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Julian Davies, Dorothy Davies
Oct 26, 2011·Antimicrobial Agents and Chemotherapy·Jeffrey B LockeKaren Joy Shaw
Dec 18, 2013·Journal of Infection in Developing Countries·Abdulrahman A AlsultanTarek T Amin
Apr 5, 2014·TheScientificWorldJournal·Rúben FernandesCristina Prudêncio

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Citations

Jun 22, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Olfa DziriChedly Chouchani
Apr 30, 2020·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Sachin Kanta RautaraySunil Kumar Ambast

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