First Detection of SARS-CoV-2 by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay in Pleural Fluid

Chest
Federico MeiStefano Gasparini

Abstract

Coronavirus disease 2019 (COVID-19) is a pandemic infection due to the spread of a novel coronavirus (severe acute respiratory syndrome coronavirus 2), resulting in a wide range of clinical features, from asymptomatic carriers to ARDS. The gold standard for diagnosis is nucleic acid detection by real-time reverse transcriptase-polymerase chain reaction in nasopharyngeal swabs. However, due to limitations in this technique's sensitivity, thoracic imaging plays a crucial, complementary role in diagnostic evaluation and also allows for detection of atypical findings and potential alternative targets for sampling (eg, pleural effusion). Although less common, pleural involvement has been described in a minority of patients. This report describes the first case of reverse transcriptase-polymerase chain reaction detection of severe acute respiratory syndrome coronavirus 2 in pleural fluid obtained by means of ultrasound-guided thoracentesis, and its main characteristics are detailed. Pleural effusion is not a common finding in COVID-19 infection, but a prompt recognition of this potential localization may be useful to optimize diagnostic evaluation as well as the management of these patients.

Citations

Sep 19, 2020·The European Respiratory Journal·José M Porcel
Oct 20, 2020·Postgraduate Medicine·David BennettBruno Frediani
Nov 6, 2020·BMJ Open Respiratory Research·Clodagh DuffyKevin G Blyth
Feb 18, 2021·Talanta·Jiaqi BuShian Zhong
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Apr 6, 2021·Journal of Pharmaceutical Analysis·Congshan JiangShemin Lu
Apr 27, 2021·Annals of Medicine and Surgery·Eko SetiawanShafira Zahra Ovaditya
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Jul 11, 2021·The European Respiratory Journal·David T ArnoldUNKNOWN AERATOR group (in alphabetical order): The AERATOR group consists of (in alphabetical order):
Dec 14, 2021·Frontiers in Molecular Biosciences·Giulia MatusaliRaffaele Strippoli

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