DOI: 10.1101/480475Dec 14, 2018Paper

First detection of tick-borne encephalitis virus in Ixodes ricinus ticks and their rodent hosts in Moscow, Russia

BioRxiv : the Preprint Server for Biology
Marat T. MakenovYanina D. Yankovskaya

Abstract

Here, we report the first confirmed autochthonous tick-borne encephalitis case diagnosed in Moscow in 2016 and describe the detection of Tick-borne encephalitis virus (TBEV) in ticks and small mammals in a Moscow park. The paper includes data from two patients who were bitten by TBEV-infected ticks within the Moscow city limits; one of these cases led to the development of the meningeal form of TBE. Both TBEV-infected ticks attacked patients in the same area. We collected ticks and trapped small mammals in this area in 2017. All samples were screened for the presence of pathogens causing tick-borne diseases by PCR. The TBEV-positive ticks and small mammals' tissue samples were subjected to virus isolation. The sequencing of the complete polyprotein gene of the positive samples was performed. A total of 227 questing ticks were collected. TBEV was detected in five specimens of Ixodes ricinus . We trapped 44 small mammals, mainly bank voles ( Myodes glareolus ) and pygmy field mice ( Apodemus uralensis ). Two samples of brain tissue from bank voles yielded a positive signal in RT-PCR for TBEV. We obtained six virus isolates from the ticks and brain tissue of a bank vole. Complete genome sequencing showed that the obtained isolates...Continue Reading

Related Concepts

Encephalitis, Central European
Tick-Borne Encephalitis
Genes
Polymerase Chain Reaction
Rodent
Ticks
Virus
Tick-Borne Infections
Viral Culture
Ixodes ricinus

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