First genetic characterization of a bacterial beta-phenylethylamine biosynthetic enzyme in Enterococcus faecium RM58

FEMS Microbiology Letters
Angela MarcobalRosario Muñoz

Abstract

Enterococcus faecium RM58 produces beta-phenylethylamine and tyramine. A gene from Ent. faecium RM58 coding for a 625 amino-acid residues protein that shows 85% identity to Enterococcus faecalis tyrosine decarboxylase has been expressed in Escherichia coli, resulting in L-phenylalanine and L-tyrosine decarboxylase activities. Both activities were lost when a truncated protein lacking 84 amino acids at its C-terminus was expressed in E. coli. This study constitutes the first genetic characterization of a bacterial protein having L-phenylalanine decarboxylase activity and solves a long-standing question regarding the specificity of tyrosine decarboxylases in enterococci.

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Citations

Nov 20, 2008·The British Journal of Nutrition·Kenneth J BroadleyWilliam R Ford
Mar 1, 2012·Critical Reviews in Food Science and Nutrition·Angela MarcobalRosario Muñoz
Jul 29, 2011·Critical Reviews in Food Science and Nutrition·Daniel M LinaresMaría Fernández
Mar 18, 2011·International Journal of Food Microbiology·Estefanía Muñoz-AtienzaCarmen Herranz
Dec 2, 2009·Pharmacology & Therapeutics·Kenneth J Broadley
May 29, 2007·International Journal of Food Microbiology·José María LandeteRosario Muñoz
Jul 5, 2016·Frontiers in Microbiology·Enrica Pessione, Simona Cirrincione
Aug 30, 2016·Frontiers in Microbiology·Fausto GardiniFatih Özogul
Jan 10, 2019·Foods·Federica BarbieriGiulia Tabanelli
Jun 27, 2018·Pharmacological Reviews·Raul R GainetdinovMark D Berry
Aug 14, 2020·Chembiochem : a European Journal of Chemical Biology·Lukas Lauterbach, Jeroen S Dickschat
Mar 6, 2010·Journal of Food Protection·M L Latorre-MoratallaM C Vidal-Carou
Dec 15, 2019·Cellular and Molecular Neurobiology·Katlynn Bugda GwiltGregory M Miller
Sep 29, 2020·Journal of Biomedical Science·Lesha Pretorius, Carine Smith
Jul 18, 2018·Frontiers in Pharmacology·Sherri L Christian, Mark D Berry
Feb 2, 2008·Journal of Food Protection·Silvia BonettaMarco Arlorio
Dec 19, 2020·Frontiers in Microbiology·Arif LuqmanFriedrich Götz
Jul 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Noreddine Benkerroum

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