First genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazil

Preventive Veterinary Medicine
Rafael Pereira HecklerFernando de Almeida Borges

Abstract

The presence of Cryptosporidium spp. in a cattle herd registered with an outbreak of diarrhea was investigated and the the molecular subtyping of Cryptosporidium parvum was characterized. Fecal samples from 85 Nellore beef cattle (Bos indicus) were collected and examined with Ziehl-Neelsen modified staining method. Fifty-four cattle (63.52%) had Cryptosporidium spp. oocysts in their feces. Fragments of genes encoding the 18S ribosomal RNA subunit and a 60-kDa glycoprotein (gp60) were amplified by nested PCR accomplished in the 11 most heavily parasitized samples, and the amplicons were sequenced. Eight of the 11 analyzed samples were positive for 18S rRNA sequences and identified monospecific infections with C. parvum. Seven samples were positive for gp60 and identified subtypes IIaA15G2R1 (6/11) and IIaA14G2R1 (1/11). This report is the first for C. parvum subtype IIaA14G2R1 in beef cattle in Brazil.

References

Dec 1, 1985·Journal of Clinical Pathology·D P CasemoreR L Sands
Jan 3, 2001·The Journal of Infectious Diseases·L XiaoA A Lal
Jan 17, 2004·Clinical Microbiology Reviews·Lihua XiaoSteve J Upton
Aug 28, 2007·Applied and Environmental Microbiology·Yaoyu FengLihua Xiao
Dec 7, 2007·International Journal for Parasitology·Peter R WielingaJoke W B van der Giessen
Dec 18, 2007·The Veterinary Journal·Emily J BrookRobert M Christley
Apr 9, 2008·Veterinary Parasitology·A BrogliaK Nöckler
Apr 11, 2009·Experimental Parasitology·Lihua Xiao
Aug 8, 2009·Veterinary Parasitology·Judit Plutzer, Panagiotis Karanis
Dec 24, 2010·Journal of Clinical Microbiology·Rongjun WangLihua Xiao
Apr 8, 2011·Parasitology Research·Marcelo V MeirelesLuiz Cláudio N Mendes
Jul 4, 2012·Journal of Applied Microbiology·C SchraderR Johne
Mar 30, 2013·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Flavio Medeiros Paz E SilvaJoão Pessoa Araújo-Junior
Apr 10, 2013·Veterinary Parasitology·Artur Rzeżutka, Agnieszka Kaupke
Nov 19, 2013·Acta Tropica·Melissa Carvalho Machado do CoutoTeresa Cristina Bergamo do Bomfim
Apr 8, 2014·Experimental Parasitology·M KoinariU M Ryan

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Citations

Jun 21, 2018·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Luciane HolsbackMércia de Seixas
Feb 23, 2019·Parasitology Research·I HolzhausenA Daugschies
Nov 28, 2020·Comparative Immunology, Microbiology and Infectious Diseases·Olufarati Oludunsin FalohunJohnson Olayide Adejinmi

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