Abstract
Five Borrelia burgdorferi sensu lato isolates from Missouri are described. This represents the first report and characterization of such isolates from that state. The isolates were obtained from either Ixodes dentatus or Amblyomma americanum ticks that had been feeding on cottontail rabbits (Sylvilagus floridanus) from a farm in Bollinger County, Mo., where a human case of Lyme disease had been reported. All isolates were screened immunologically by indirect immunofluorescence by using monoclonal antibodies to B. burgdorferi-specific outer surface protein A (OspA) (antibodies H3TS and H5332), B. burgdorferi-specific OspB (antibody H6831), Borrelia (genus)-specific antiflagellin (antibody H9724), and Borrelia hermsii-specific antibody (antibody H9826). Analysis of the isolates also involved a comparison of their protein profiles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Finally, the isolates were analyzed by PCR with six pairs of primers known to amplify selected DNA target sequences specifically found in the reference strain B. burgdorferi B-31. Although some genetic variability was detected among the five isolates as well as between them and the B-31 strain, enough similarities were found to classify them a...Continue Reading
References
Dec 1, 1992·The American Journal of Tropical Medicine and Hygiene·B J JohnsonJ Piesman
Jun 5, 1992·Science·R N Brown, R S Lane
Mar 1, 1990·Journal of Clinical Microbiology·D H PersingS W Barthold
Sep 1, 1985·The Journal of Infectious Diseases·A G BarbourT R Howe
Dec 1, 1989·The Journal of Infectious Diseases·P A Rosa, T G Schwan
Aug 1, 1989·Journal of Clinical Microbiology·R J Sinsky, J Piesman
Jan 1, 1989·Journal of Clinical Microbiology·J F AndersonR C Johnson
Aug 15, 1970·Nature·U K Laemmli
Dec 1, 1984·Journal of Clinical Microbiology·R C JohnsonC Kodner
Sep 1, 1995·Journal of Clinical Microbiology·R T MarconiI Schwartz
Aug 1, 1995·The Journal of Infectious Diseases·G L CampbellD T Dennis
Feb 1, 1996·The Journal of Infectious Diseases·A G BarbourJ Piesman
Jun 1, 1996·The Journal of Infectious Diseases·E J Masters, H D Donnell
Mar 1, 1996·Journal of Clinical Microbiology·J F AndersonE Fikrig
Jan 1, 1997·The Journal of Infectious Diseases·D A MathiesenD H Persing
Citations
Jul 20, 1999·Current Opinion in Rheumatology·J Evans
Aug 18, 2004·Journal of Medical Entomology·A S VarelaS E Little
Mar 9, 2005·Journal of Applied Microbiology·T L CyrG A McDonald
Dec 11, 1999·Journal of Medical Entomology·T M KollarsL A Durden
Oct 27, 1999·Journal of Medical Entomology·J H OliverJ F Anderson
Jun 22, 2002·The Pediatric Infectious Disease Journal·Edwin Masters, Charles Crist
Feb 25, 2003·Journal of Medical Entomology·Thomas M Kollars, James H Oliver
Jul 31, 2001·Journal of Medical Entomology·J D ScottM G Morshed
Dec 19, 2003·Journal of Medical Entomology·Rendi Murphree BaconBarbara J B Johnson
Mar 28, 2001·Journal of Medical Entomology·E Y StromdahlA G Gutierrez
Feb 10, 2006·Journal of Medical Entomology·A J UllmannJ Piesman
Jan 25, 2011·Applied and Environmental Microbiology·Sarah A HamerJean I Tsao
Aug 9, 2006·Journal of Medical Entomology·M G MorshedL A Durden
Apr 8, 2004·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·Tomasz ChmielewskiStanislawa Tylewska-Wierzbanowska
Aug 26, 2011·The Journal of Parasitology·John D ScottLance A Durden
Jun 7, 2007·Medical and Veterinary Entomology·S M ToddW L Hynes
Dec 12, 2012·Applied and Environmental Microbiology·Nataliia RudenkoJames H Oliver
Apr 5, 2002·Journal of Medical Entomology·Kerry L ClarkCraig W Banks
Nov 6, 2004·Journal of Clinical Microbiology·Kerry Clark
Mar 9, 2004·Journal of Clinical Microbiology·Andrea S VarelaSusan E Little
Dec 10, 2003·Journal of Clinical Microbiology·Ellen Y StromdahlNicole A Dobbs
Nov 21, 2008·Journal of Clinical Microbiology·Nataliia RudenkoJames H Oliver
Oct 23, 2009·Journal of Clinical Microbiology·Nataliia RudenkoJames H Oliver
Jun 28, 2001·Journal of Clinical Microbiology·T LinK L Clark
Jan 5, 2000·Journal of Clinical Microbiology·J H OliverL A Durden