First isolation and structural determination of cyclic beta-(1-->2)-glucans from an alga, Chlorella pyrenoidosa

Carbohydrate Research
Erick Reyes SuárezT Bruce Grindley

Abstract

The aqueous extract of the edible green microalgae Chlorella pyrenoidosa is of interest because of its immunostimulatory activity. Some components in the extract have been identified previously, namely a unique type of arabinogalactan and a galactofuran. Further fractionation of this extract was accomplished by treating the aqueous solution of the fraction precipitated by addition of 1.5vol of 95% ethanol with cetyltrimethylammonium bromide. The residue obtained by concentration of the supernatant was fractionated further by anion-exchange chromatography and size-exclusion chromatography on Sephadex G-100. Two fractions from the latter column were retained, of which one was a starch-like alpha-(1-->4)-linked d-glucan with some alpha-(1-->6) branches, and the other contained a starch plus a mixture of beta-(1-->2)-d-glucans. ESI mass spectrometry was used to show that the mixture contained both cyclic and linear beta-(1-->2)-d-glucans in a cyclic:linear ratio of 64:36, based on intensities of mass spectral peaks. For the cyclic beta-(1-->2)-d-glucans, ring sizes ranged from 18 to 35 monosaccharides with the ring containing 21 glucose units (54% of the cyclic glucans) being greater than three times more abundant than the next mos...Continue Reading

References

Jan 27, 2000·International Journal of Colorectal Disease·G JacobaschK Schmehl
Apr 26, 2000·FEMS Microbiology Letters·J P Bohin
Dec 28, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·J A KralovecH S Ewart
Jul 3, 2007·The Plant Cell·Luciano Ariel RiganoAdrian Alberto Vojnov

❮ Previous
Next ❯

Citations

Nov 4, 2009·European Journal of Nutrition·Jiezhong Chen, Xu-Feng Huang
Jul 25, 2013·Journal of Asian Natural Products Research·Xiao-Qiang HanPeng-Fei Tu
Sep 1, 2012·Carbohydrate Polymers·Zhongquan SuiJames N BeMiller
Jul 4, 2012·International Journal of Biological Macromolecules·Xiao-Qiang HanQuan-Bin Han
Nov 20, 2016·International Journal of Biological Macromolecules·Jia Qi, Sang Moo Kim
Jan 30, 2010·Natural Product Reports·John W BluntMichèle R Prinsep
Jun 14, 2019·Bioscience, Biotechnology, and Biochemistry·Kaito KobayashiHayao Taguchi
Jul 30, 2021·Critical Reviews in Biotechnology·Guilherme Augusto ColusseMiguel Daniel Noseda
Dec 28, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A I Ruiz-MatuteI Martínez-Castro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.