First isolation of Cryptococcus neoformans genotype VNI MAT-alpha from wood inside hollow trunks of Hymenaea courbaril

Medical Mycology
D M Castro e SilvaM S C Melhem

Abstract

Cryptococcal infection is transmitted by the inhalation of Cryptococcus spp. propagules. Information about the Cryptococcus species inhabiting plants might be clinically relevant due to the epidemiological role of these habitats as possible sources of human infection. The aim of this study was to increase the knowledge about the environmental occurrence of cryptococcosis agents. Hollow tree vegetal debris of nine plant species was sampled quarterly over a 12-month period. Melanized colonies were screened for Cryptococcus neoformans and C. gattii by biochemical tests, followed by URA5-RFLP molecular analysis, M13 fingerprinting assays, and mating-typing with the specific a and α primers. The susceptibility to fluconazole of all of the confirmed species colonies was determined using the AFST-EUCAST broth dilution method. We found that the typical Brazilian flora tree Hymenaea courbaril yielded a high cryptococcal burden (median, 10(2) CFU/g) during the summer, autumn and winter seasons. C. neoformans VNI molecular type MAT alpha was identified in all of the samples. The fingerprinting analyses showed great molecular variability with no correlation with the susceptibility profile to fluconazole (MIC range 4 to ≥64 mg/l). To our kn...Continue Reading

References

Jan 1, 1992·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·T J Pfeiffer, D H Ellis
Jan 1, 1993·Revista Do Instituto De Medicina Tropical De São Paulo·C C MachadoL C Severo
Nov 25, 2000·Medical Mycology·M S LazeraB Wanke
Feb 27, 2003·Emerging Infectious Diseases·Wieland MeyerUNKNOWN IberoAmerican Cryptococcal Study Group
May 16, 2006·FEMS Yeast Research·Kyung J Kwon-Chung, Ashok Varma
Jun 20, 2007·Mycoses·Juliana Quero ReimãoAntônio Martins de Siqueira
Sep 2, 2011·Trends in Microbiology·Vishnu Chaturvedi, Sudha Chaturvedi
May 9, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Maiken C ArendrupUNKNOWN EUCAST-AFST
Feb 28, 2015·Fungal Genetics and Biology : FG & B·Ferry HagenTeun Boekhout

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Citations

Feb 18, 2016·International Journal of Microbiology·Mahmoud ElhaririMohamed Refai
Jul 28, 2020·Journal of Fungi·Man Shun Fu, Rebecca A Drummond
Jul 16, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Denise de Oliveira ScoarisCarlos Augusto Rosa

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