First isolation of Toxoplasma gondii from cats of Colima, Mexico: tissue distribution and genetic characterization

Veterinary Parasitology
Claudia Patricia Rico-TorresJosé Manuel Palma-García

Abstract

Toxoplasma gondii is among the commonest zoonotic infectious agents worldwide. It infects many warm-blooded animals, including felines, the definitive hosts. This parasite is now classified in 15 haplogroups spread out around the world. Few reports reveal a predominance of genotypes I and III in Mexico, although recombinant and atypical variants have also been found in humans and animals. The aim of this study was to detect, isolate and genotype T. gondii from cats of Colima Mexico, and to analyze tissue distribution of the parasite. IgG specific antibodies were investigated in 48 serum samples from unwanted and stray cats by indirect ELISA. Isolation in mice and molecular characterization by PCR-RFLP and sequencing were attempted using pools of brain, heart, liver, lung, spleen and brachiocephalic muscle samples of seropositive cats. Fourteen animals (29.2%) were seropositive, the frequency ranged between 27.3 and 40% among the different localities. Ten seropositive animals were euthanized, eight of them were positive for the B1 gene by conventional PCR. More frequently infected tissues were the brachiocephalic muscle (75.0%) the brain (63.0%) and the spleen (63.0%). Genotype III was determined for the SAG3 locus of the parasi...Continue Reading

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Dec 17, 2011·The Pediatric Infectious Disease Journal·Claudia Patricia Rico-TorresDolores Correa
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chunlei SuL David Sibley

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Citations

May 23, 2017·Vector Borne and Zoonotic Diseases·Williams Arony Martínez-FloresPablo Maravilla
Aug 30, 2019·Transboundary and Emerging Diseases·Afsaneh AmoueiAhmad Daryani
Mar 14, 2019·Frontiers in Veterinary Science·Rafael Calero-Bernal, Solange M Gennari
Jul 10, 2020·Veterinary Parasitology·J P DubeyC Su
May 28, 2021·International Journal for Parasitology. Parasites and Wildlife·Sawsan AmmarRichard Gerhold

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