First molecular subtyping and phylogeny of Blastocystis sp. isolated from domestic and synanthropic animals (dogs, cats and brown rats) in southern Iran.

Parasites & Vectors
Iraj MohammadpourNiloofar Mohammadpour

Abstract

Blastocystis sp. is a common intestinal protist that infects humans and many animals globally. Thus far, 22 subtypes (STs) have been identified in mammalian and avian hosts. Since various STs are common to humans and animals, it was suggested that some human infections might arise from zoonotic transmission. Therefore, the aim of this study was to assess the presence of Blastocystis sp. in domestic (dogs and cats) and synanthropic animals (rats) of Fars Province, Iran, and to genetically characterize the samples. A total of 400 fresh faecal samples from 154 dogs, 119 cats, and 127 rats were inspected by direct microscopy, Wheatley's trichrome staining, in vitro culture, and 18S rRNA gene nested-PCR. Finally, sequencing and phylogenetic analyses were performed. Out of 400 samples, 47 (11.8%) and 61 (15.3%) samples were detected as positive by direct wet mount and culture, respectively. Molecular analysis detected a larger number of positive samples (n = 70, 17.5%): nested-PCR showed that 29 (18.8%) dogs, 21 (17.7%) cats, and 20 (15.8%) rats were infected by Blastocystis sp. Sequence analysis of positive samples indicated the presence of zoonotic STs in all investigated host species. Specifically, ST2 (allele 9), ST3 (allele 34),...Continue Reading

References

Jun 14, 2002·Veterinary Parasitology·Niichiro AbeHisao Yoshikawa
Jan 15, 2005·Journal of Wildlife Diseases·S Solaymani-MohammadiM A Anwar
Sep 10, 2005·Applied and Environmental Microbiology·K SureshT C Tan
Feb 16, 2007·The Journal of Eukaryotic Microbiology·Hisao YoshikawaKevin S W Tan
Aug 1, 2007·Parasitology Research·Yiming YanXiaobing Luo
Aug 30, 2008·International Journal for Parasitology·C Rune StensvoldC Graham Clark
Nov 18, 2008·Trends in Parasitology·C Rune StensvoldHuw V Smith
Jan 22, 2010·Veterinary Parasitology·Unaiza ParkarR C Andrew Thompson
Feb 5, 2011·The American Journal of Tropical Medicine and Hygiene·Tamalee RobertsDamien Stark
Jun 3, 2011·The American Journal of Tropical Medicine and Hygiene·Christen Rune StensvoldHenrik Vedel Nielsen
Nov 15, 2011·Parasitology Research·I L LeeK G Suresh
Nov 26, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·C Rune StensvoldC Graham Clark
Jun 30, 2012·Parasites & Vectors·Li Ii LeeSuresh Kumar Govind
Sep 11, 2012·Parasitology Research·Tian Chye TanKumar Govind Suresh
Jan 8, 2013·Acta Tropica·Mohammed A AlfellaniC Graham Clark
Apr 4, 2013·Advances in Parasitology·C Graham ClarkC Rune Stensvold
Apr 9, 2013·Parasitology·Mohammed A AlfellaniC Rune Stensvold
Jun 19, 2013·Protist·Mohammed A AlfellaniC Graham Clark
Jul 26, 2013·Parasites & Vectors·Wenqi WangRebecca J Traub
Jul 28, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Juan David RamírezChristen Rune Stensvold
Mar 29, 2014·BMC Infectious Diseases·Dima El SafadiEric Viscogliosi
May 13, 2015·Jundishapur Journal of Microbiology·Ebrahim BadparvaFarnaz Kheirandish
Feb 24, 2016·Parasitology International·Maria Luz B BellezaWindell L Rivera
Apr 2, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Juan David RamírezRodolfo D Casero
Apr 20, 2016·Parasitology International·Hisao YoshikawaKazutoshi Takami
May 18, 2016·Parasitology International·Sitara S R Ajjampur, Kevin S W Tan
Nov 20, 2016·Current Protocols in Microbiology·C Graham Clark, C Rune Stensvold
Mar 23, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M LepczyńskaJ Korycińska

❮ Previous
Next ❯

Citations

May 1, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Morteza ShamsSoheil Hassanipour
May 15, 2021·Veterinary Research Communications·Xuehan LiuLongxian Zhang
Aug 12, 2021·Clinical Microbiology Reviews·Simone MorelliDonato Traversa

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR
electrophoresis

Software Mentioned

MEGA
nBLAST
Chromas
GraphPad Prism
BioEdit

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Parasitology Research
Adedolapo Aminat Rauff-AdedotunMeor Termizi Farah Haziqah
European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
J ForsellB Evengård
© 2021 Meta ULC. All rights reserved