First natural crossover recombination between two distinct species of the family Closteroviridae leads to the emergence of a new disease

PloS One
Leticia RuizDirk Janssen

Abstract

Lettuce chlorosis virus-SP (LCV-SP) (family Closteroviridae, genus Crinivirus), is a new strain of LCV which is able to infect green bean plants but not lettuce. In the present study, high-throughput and Sanger sequencing of RNA was used to obtain the LCV-SP full-length sequence. The LCV-SP genome comprises 8825 nt and 8672 nt long RNA1 and RNA2 respectively. RNA1 of LCV-SP contains four ORFs, the proteins encoded by the ORF1a and ORF1b are closely related to LCV RNA1 from California (FJ380118) whereas the 3´ end encodes proteins which share high amino acid sequence identity with RNA1 of Bean yellow disorder virus (BnYDV; EU191904). The genomic sequence of RNA2 consists of 8 ORFs, instead of 10 ORFs contained in LCV-California isolate. The distribution of vsiRNA (virus-derived small interfering RNA) along the LCV-SP genome suggested the presence of subgenomic RNAs corresponding with HSP70, P6.4 and P60. Results of the analysis using RDP4 and Simplot programs are the proof of the evidence that LCV-SP is the first recombinant of the family Closteroviridae by crossover recombination of intact ORFs, being the LCV RNA1 (FJ380118) and BnYDV RNA1 (EU191904) the origin of the new LCV strain. Genetic diversity values of virus isolates i...Continue Reading

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Citations

Sep 1, 2019·Viruses·Lior HadadAviv Dombrovsky
Nov 3, 2020·AIMS Microbiology·Sergey Y Morozov, Andrey G Solovyev
Feb 2, 2021·Molecular Plant-microbe Interactions : MPMI·Soledad SacristánSebastian Eves-van den Akker
Oct 10, 2021·Molecular Plant-microbe Interactions : MPMI

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Datasets Mentioned

BETA
MG489894
EU191905
KX685959
KY430285
KX685958
KY430286
FJ380119

Methods Mentioned

BETA
PCR

Software Mentioned

jModelTest
GENECONV
SimPlot
BLASTN
BOOTSCAN
MaxChi
MrBayes
MISIS
MEGA
RDP4

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