First profiling of lysine crotonylation of myofilament proteins and ribosomal proteins in zebrafish embryos

Scientific Reports
Oh Kwang KwonSangkyu Lee

Abstract

Zebrafish embryos are translucent and develop rapidly in individual eggs ex utero; they are widely used as models for embryogenesis and organ development for human diseases and drug discovery. Lysine crotonylation (Kcr) is a type of histone post-translational modifications discovered in 2011. Kcr dynamics are involved in gene expression regulation and acute kidney injury; however, little is known about the effects of Kcr on non-histone proteins. In the present study, we conducted the first proteome-wide profiling of Kcr in zebrafish larvae and identified 557 Kcr sites on 218 proteins, representing the Kcr event in zebrafish. We identified two types of Kcr motifs containing hydrophobic (Leu, Ile, Val) and acidic (Asp and Glu) amino acids near the modified lysine residues. Our results show that both crotonylated proteins and sites of crotonylation were evolutionarily conserved between zebrafish embryos and humans. Specifically, Kcr on ribosomal proteins and myofilament proteins, including myosin, tropomyosin and troponin, were widely enriched. Interestingly, 55 lysine crotonylation sites on myosin were distributed throughout coiled coil regions. Therefore, Kcr may regulate muscle contraction and protein synthesis. Our results pro...Continue Reading

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Citations

Aug 31, 2020·Proteomics·Jiang-Feng LiuJun-Tao Yang
Sep 3, 2019·Journal of Cellular and Molecular Medicine·Junhu WanHongquan Zhang
Nov 17, 2020·Frontiers in Microbiology·Ning ZhangMengjie Liu
Feb 8, 2021·Epigenetics & Chromatin·Kun Li, Ziqiang Wang
Mar 7, 2021·International Journal of Molecular Sciences·Kathiresan PurushothamanShubha Vij
Apr 30, 2021·Journal of Molecular and Cellular Cardiology·Ashley FrancoisMatthew S Stratton
Jun 2, 2021·Cell & Bioscience·Jia-Yi HouJi-Min Cao
Jul 6, 2021·Canadian Respiratory Journal : Journal of the Canadian Thoracic Society·Qing GanYong Dai
Jul 16, 2021·Cell Death & Disease·Gaoyue JiangHuihui Li
Sep 5, 2019·Journal of Proteome Research·Yan GaoSangkyu Lee
Sep 29, 2021·Omics : a Journal of Integrative Biology·Pratigya Subba, Thottethodi Subrahmanya Keshava Prasad

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Methods Mentioned

BETA
acetylation
immunoprecipitation
electrophoresis
ubiquitination
deamidation

Software Mentioned

Functional Annotation Tool of
PhosphoSitePlus
- X
PSORT
Functional Annotation Tool of DAVID Bioinformatics Resources
BlastP
KAAS
KEGG mapper
MaxQuant
PSI

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