First Report of a Fatal Case Associated with EV-D68 Infection in Hong Kong and Emergence of an Interclade Recombinant in China Revealed by Genome Analysis

International Journal of Molecular Sciences
Cyril C Y YipSusanna K P Lau

Abstract

A fatal case associated with enterovirus D68 (EV-D68) infection affecting a 10-year-old boy was reported in Hong Kong in 2014. To examine if a new strain has emerged in Hong Kong, we sequenced the partial genome of the EV-D68 strain identified from the fatal case and the complete VP1, and partial 5'UTR and 2C sequences of nine additional EV-D68 strains isolated from patients in Hong Kong. Sequence analysis indicated that a cluster of strains including the previously recognized A2 strains should belong to a separate clade, clade D, which is further divided into subclades D1 and D2. Among the 10 EV-D68 strains, 7 (including the fatal case) belonged to the previously described, newly emerged subclade B3, 2 belonged to subclade B1, and 1 belonged to subclade D1. Three EV-D68 strains, each from subclades B1, B3, and D1, were selected for complete genome sequencing and recombination analysis. While no evidence of recombination was noted among local strains, interclade recombination was identified in subclade D2 strains detected in mainland China in 2008 with VP2 acquired from clade A. This study supports the reclassification of subclade A2 into clade D1, and demonstrates interclade recombination between clades A and D2 in EV-D68 stra...Continue Reading

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Citations

Feb 2, 2018·International Journal of Molecular Sciences·Susanna K P Lau
Jan 24, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Antonin BalLaurence Josset
Oct 3, 2017·The Journal of Infectious Diseases·Alison M HixonKenneth L Tyler
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Jun 22, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Shi-Huan TangNan Yu
Jul 10, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yulu FangChangzheng Dong
Oct 1, 2021·Microbiology Resource Announcements·Michiko OkamotoHitoshi Oshitani
Aug 20, 2021·Virulence·Syriam Sooksawasdi Na AyudhyaDebby van Riel

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Datasets Mentioned

BETA
EV-D68
KY767820
KY767842

Methods Mentioned

BETA
PCR
AFM

Software Mentioned

GENECONV
Bootscan
Recombination Detection Program
MEGA6
BioEdit
MatGAT2
SiScan
SimPlot
ClustalX

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