First Report of Aleurocanthus spiniferus on Ailanthus altissima : Profiling of the Insect Microbiome and MicroRNAs

Insects
Giovanni BubiciFrancesco Porcelli

Abstract

We report the first occurrence of the orange spiny whitefly (Aleurocanthus spiniferus; OSW) on the tree of heaven (Ailanthus altissima) in Bari, Apulia region, Italy. After our first observation in 2016, the infestation recurred regularly during the following years and expanded to the neighboring trees. Since then, we have also found the insect on numerous patches of the tree of heaven and other plant species in the Bari province. Nevertheless, the tree of heaven was not particularly threatened by the insect, so that a possible contribution by OSW for the control of such an invasive plant cannot be hypothesized hitherto. This work was also aimed at profiling the microbiome of OSW feeding on A. altissima. For this purpose, we used the denaturing gradient gel electrophoresis (DGGE) and the deep sequencing of small RNAs (sRNAs). Both techniques unveiled the presence of "Candidatus Portiera" (primary endosymbiont), Wolbachia sp. and Rickettsia sp., endosymbionts already reported for other Aleyrodidae. Deep sequencing data were analyzed by four computational pipelines in order to understand the reliability of the detection of fungi, bacteria, and viruses: Kraken, Kaiju, Velvet, and VelvetOptimiser. Some contigs assembled by Velvet o...Continue Reading

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Methods Mentioned

BETA
sRNA-Seq
electrophoresis
PCR

Software Mentioned

miRDeep2
bowtie
Galaxy
OSW
MEGAN6
Collapse sequences
BLAST
FASTQC
isomiRID
VelvetOptimiser

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