First Report of Bacterial Wilt of Annual Bluegrass Caused by Xanthomonas translucens pv. poae in Montana

Plant Disease
Nathaniel Mitkowski

Abstract

During August 2003, a golf course putting green sample composed of Poa annua from the Buffalo Hill Country Club in Kalispell, MT exhibiting symptoms of general decline, wilting, and necrosis was submitted to the University of Rhode Island Turfgrass Disease Diagnostic Laboratory. No pathogenic fungi were observed or cultured from affected plants. Bacterial streaming was observed from cut leaves. Cut leaves were surface disinfested for 5 min in a 0.6% sodium hypochlorite solution and plated on yeast dextrose calcium carbonate (YDC) agar media. A single yellow, mucoid colony type composed of rod-shaped bacteria was isolated from all leaves. Bacteria were gram negative, lacked anaerobic growth, did not fluorescence on King's medium B, and were able to grow at 33°C on YDC. Colonies were transferred to YDC for 10 days, DNA was extracted and a 2,190-bp region encompassing the 16S rRNA, ITS, and 5' end of the 23S rRNA was amplified via polymerase chain reaction (PCR) using previously published protocols (1). Sequence comparisons of the resulting 2,190-bp PCR product revealed a 99.7% sequence similarity with X. translucens pv. poae (American Type Culture Collection [ATCC] no. 33804) and a 99.8% sequence similarity with X. translucens pv...Continue Reading

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