First Report of Charcoal Rot on Soybean Caused by Macrophomina phaseolina in North Dakota

Plant Disease
C A Bradley, L E Del Río

Abstract

In late August 2002, patches of soybean (Glycine max) plants in a field in Richland County, ND exhibited symptoms and signs of charcoal rot and died prematurely. Dead plants had a silvery-gray appearance, and black microsclerotia (76 ± 28 μm in diameter) were present in the vascular tissue of the lower stems and roots. Stem tissue was placed in petri dishes containing potato dextrose agar (PDA). Gray hyphae grew from the stem tissue and subsequently turned black and formed microsclerotia (188 × 139 ± 48 μm). The fungus was identified as Macrophomina phaseolina (Tassi) Goid. based on colony color and morphology and size of the microsclerotia (1). To confirm pathogenicity, soybean plants (cv. Garst D 041 RR) were grown in the greenhouse and inoculated with the previously obtained isolate of M. phaseolina. Stems of soybean plants at the V2 stage were excised just below the third node. Mycelia plugs of a 1-week-old culture of M. phaseolina were placed into the large end of disposable micropipette tips (200 μl). The micropipette tips containing the M. phaseolina culture were subsequently placed over 10 excised soybean stems. To serve as a control, 10 excised soybean stems were inoculated with micropipette tips containing plugs of no...Continue Reading

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