First report of mecC MRSA in human samples from Austria: molecular characteristics and clinical data

New Microbes and New Infections
H KerschnerP Apfalter

Abstract

Reports of mecC methicillin-resistant Staphylococcus aureus (MRSA) strains have been published from several European countries. We describe the first six mecC MRSA isolates of human origin from Austria and report the application of a rapid PCR test. Candidate isolates (n = 295) received between 2009 and 2013 were investigated phenotypically by cefoxitin screening and streaking on ChromID MRSA plates. The presence of mecC was confirmed in six isolates from blood cultures, wound swabs and screening samples of four female and two male patients (age range 7-89 years) by an in-house PCR method and the new Genspeed MRSA test (Greiner Bio-One, Kremsmünster, Austria). The mecC MRSA were further characterized by whole genome sequencing, multilocus sequence and spa typing. Antimicrobial susceptibility testing was performed by Eucast disk-diffusion method and Vitek 2. The six mecC MRSA isolates were from two clonal lineages (CC130, including a new single-locus variant, and CC599) and four different spa types (t843, t1535, t3256, t5930). Analysis for virulence factor genes yielded lukED, eta, etd2 and edin-B (CC130 isolates) and tst, lukED, eta and sel (ST599 isolates). The Genspeed MRSA test identified mecC in all isolates whereas Vitek 2...Continue Reading

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Citations

Aug 8, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Alessio BortolamiDorina Timofte
Feb 25, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Lucia MališováHelena Žemličková
Jan 31, 2020·Journal of Clinical Microbiology·Karen C CarrollFrederick S Nolte
Sep 14, 2018·Clinical Microbiology Reviews·Sahreena Lakhundi, Kunyan Zhang
Aug 8, 2020·Transboundary and Emerging Diseases·José Givanildo SilvaRinaldo Aparecido Mota
Oct 24, 2020·Microorganisms·Carmen LozanoCarmen Torres

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Datasets Mentioned

BETA
ERR
490434
ERR490433

Methods Mentioned

BETA
PCR
chip
electrophoresis

Software Mentioned

Vitek

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