First Report of Natural Infection of Luffa acutangula by Watermelon bud necrosis virus in India

Plant Disease
B MandalA Varma

Abstract

In August 2002, ridge gourd (Luffa acutangula) plant samples exhibiting yellowing of leaves were collected from the experimental farm of Tamil Nadu Agricultural University, Coimbatore, India. Mechanical inoculations of ridge gourd seedlings using the above samples resulted in chlorotic spots on inoculated leaves and vein clearing followed by chlorotic rings and yellow netting along veins of newly developed leaves. The virus was reisolated from the chlorotic spots of the seedlings. Extracts from the field samples as well as mechanically inoculated seedlings reacted with antiserum to Watermelon silver mottle virus (WSMoV) in direct antigen coating enzyme-linked immunosorbent assay, suggesting the association of a tospovirus belonging to WSMoV serogroup (2). Symptomatic leaves from the second mechanical passage exhibiting yellow netting along the veins were subjected to reverse transcription-polymerase chain reaction (1) to identify the genus Tospovirus. Using the primer pair (5'TCTGTCCT(C/T) TTGAA (G/T) GTCCA3' and 5'AGAGCAATCGAGGCGCT3') derived from the conserved sequences of Groundnut bud necrosis virus (GBNV) and WSMoV, part of the nucleocapsid (N) protein gene and the complete noncoding region upstream of the N gene's coding ...Continue Reading

Citations

Feb 9, 2011·Phytopathology·Suresh R KunkalikarKankanallu S Ravi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adenocarcinoma, Clear Cell

Clear Cell Adenocarcinoma is a tumor that arises in the female genital tract and is characterized by cells that appear clear under the microscope. Discover the latest research here.