First Report of NDM and VIM Coproducing Klebsiella pneumoniae in Tunisia and Emergence of Novel Clones

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Raoudha DziriNaouel Klibi

Abstract

Metallo-β-lactamase (MBL) producing bacteria constitute nowadays a serious global concern worldwide. The purpose of our present study was to characterize molecular features of MBL producing bacteria and to identify the existing clones in our area. Thirteen MBL-producing-Klebsiella pneumoniae were detected in clinical samples from patients hospitalized in the Military hospital of Tunisia during 2017. The molecular research by polymerase chain reaction and sequencing of gene encoding MBL enzymes showed that only two types were identified in our study: blaNDM-1 and blaVIM-1 genes detected, respectively, in eight and six isolates. An association between these two MBL genes (blaNDM-1+blaVIM-1) has been observed in one of our isolates. Other β-lactamase types (CTXM-15/4 isolates; SHV/2 isolates; OXA-48/3 isolates) were detected in association with New Delhi metallo-beta-lactamase (NDM) and/or Verona Integron-Mediated Metallo-β-lactamase (VIM) enzymes. Furthermore, these isolates were resistant to other antimicrobial agents, including gentamicin [aac(3)-II/11 isolates], tetracycline (tetB or tetA/2 isolates), chloramphenicol (cmlA and/or floR/3 isolates), streptomycin (aadA/5 isolates), and sulfonamides (sul1 or sul2 or sul3/4isolates...Continue Reading

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Citations

Apr 14, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Claudia FloresCélia Maria C P A Romão
Aug 5, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Raoudha DziriNaouel Klibi
Dec 4, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Larbi Zakaria NabtiJean-Marc Rolain

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Methods Mentioned

BETA
PCR

Software Mentioned

Morroco

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