First report of qacG, qacH and qacJ genes in Staphylococcus haemolyticus human clinical isolates

The Journal of Antimicrobial Chemotherapy
J E CorreaP E Jeric

Abstract

To investigate phenotypically and genotypically the presence of MDR efflux pumps in 21 clinical isolates of Staphylococcus haemolyticus collected over a period of 10 years. MICs of different antibiotics and biocides were determined by the broth dilution method in the presence/absence of carbonyl cyanide-m-chlorophenylhydrazone (CCCP), an efflux pump inhibitor. PCR followed by sequencing was performed to detect the qac genes that encode for antiseptic resistance. Clonal relationships were determined by PFGE SmaI patterns using a standard protocol. All the isolates were resistant to gentamicin, 15 to erythromycin, 18 to ciprofloxacin, 7 to chloramphenicol and 1 to tetracycline. They showed higher susceptibility to antibiotics when they were exposed to CCCP. The MICs of ethidium bromide, SDS and benzalkonium chloride were also decreased, whereas the MIC of triclosan was decreased in only four isolates in the presence CCCP. Of the 21 isolates, qacA/B was detected in 5 isolates, smr in all of the isolates, qacG in 11 isolates, qacH in 10 isolates and qacJ in 4 isolates. PFGE analysis of the 21 isolates clustered them into 14 clones at 90% similarity corresponding to differences of between 7 and 16 bands among the clones. The efflux ...Continue Reading

References

Jun 25, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammed Mahbub AlamNaoki Watanabe
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Citations

Jul 21, 2011·Asian Pacific Journal of Tropical Medicine·Fengying HuangHaofu Dai
Apr 8, 2010·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Kristin HegstadSiamak P Yazdankhah
Oct 25, 2011·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Meike BischoffChristina Hölzel
Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Jan 31, 2015·International Journal of Environmental Research and Public Health·Jody L AndersenManuel F Varela
Dec 12, 2012·Expert Opinion on Investigational Drugs·Divya Prakash GnanadhasDipshikha Chakravortty
Oct 30, 2015·International Journal of Food Microbiology·Xiaobing JiangLijun Zhou
Oct 3, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Maria Amalie Seier-PetersenYvonne Agersø
Apr 18, 2015·European Journal of Microbiology & Immunology·Trudy M WassenaarHanne Ingmer
Sep 14, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Sara Hernando-AmadoJosé L Martínez
Mar 4, 2014·Journal of Medical Microbiology·Gui-Xin HeHongmiao Pan

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