First Report of Shining Willow as a Host Plant for Septoria musiva

Plant Disease
N Feau, L Bernier

Abstract

During the summer of 2001, leaf spots resembling those caused by Septoria musiva Peck. were observed on shining willow (Salix lucida Mühl. subsp. lucida) at Leclerville, Québec, Canada (46°34'19″N,71°59'35″W). Affected leaves had brown, necrotic leaf spots (>5mm in diameter) surrounded by a darker brown halo. Conidia were cylindrical, straight to curved with 1 to 4 septa, 28 to 54 × 3.5 to 4 μm, and were produced in pycnidia located on the abaxial surface in the center of the leaf spots. The causal agent of this disease was successfully isolated by germinating the conidia on corn meal agar that was supplemented with streptomycin (50mg/ml) and chloramphenicol (300mg/ml) and followed with the transfer of the germinated conidia to potato dextrose agar. Leaf symptoms and morphology matched those of S. musiva, the cause of leaf spot and stem canker of hybrid poplars in North America (2,4). The internal transcribed spacers and the 5.8S portion of the rDNA were amplified using PCR with the ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3') and ITS2 (5'-GCT GCG TTC TTC ATC GAT GC-3') primer pair on total genomic DNA extracted from a pure culture of the pathogen. The rDNA sequence obtained (GenBank Accession No. AY555277) had 100% identity at 506 b...Continue Reading

Citations

Apr 11, 2018·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (EFSA PLH Panel)Marco Pautasso

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