First Report of the Association of a Nanovirus with Foorkey Disease of Large Cardamom in India

Plant Disease
Bikash MandalAnupam Varma

Abstract

Large cardamom (Amomum subulatum Roxb.) is affected by the serious disease 'foorkey', which is of unknown etiology (1). Excessive sprouting and formation of bushy dwarf clumps at the base of mother plants that gradually die, characterize the disease. In surveys in the Sikkim-Darjeeling Hill area during 2002 and 2003, as much as 27% of plants were found to be affected. Electron microscopy of negatively stained, partially purified preparations from field-infected large cardamom plants showed the presence of isometric particles measuring 17 to 20 nm. The causal agent was successfully transmitted by the aphid Micromyzus kalimpongensis Basu, in a persistent manner. Ten randomly selected field samples gave A405 0.1 to 0.23 compared with 0.57 with positive control with enzyme-linked immunosorbent assay using antiserum to an Indian isolate of Banana bunchy top virus (BBTV). A fragment of approximately 850 bp was amplified from the diseased plants using polymerase chain reaction (PCR) with primers 5'-ATGGCGCGATATGTGGTATGC-3' and 5'-TCAGCAAGAAAACCAACTTTATTC-3', designed to amplify the putative Rep gene based on the sequences of DNA1 of BBTV. The PCR product was cloned and sequenced (GenBank Accession No. AY 485960) and showed 80 to 82% i...Continue Reading

Citations

Jun 1, 2010·Indian Journal of Virology : an Official Organ of Indian Virological Society·Bikash Mandal
Jun 20, 2014·Evolutionary Applications·Fiona R SavoryUma Ramakrishnan
Jan 14, 2010·Archives of Virology·Adane D AbrahamH Josef Vetten
Aug 7, 2009·Journal of Virology·Ioana GrigorasBruno Gronenborn
Dec 18, 2020·Frontiers in Plant Science·Aamir LalSukchan Lee

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