First Report of the Occurrence of Tomato chlorosis virus and Tomato infectious chlorosis virus in Taiwan

Plant Disease
W S TsaiH Y Liu

Abstract

Pronounced yellowing symptoms on the lower leaves of tomato plants, similar to those caused by nitrogen deficiency, were observed in the spring of 1998 in The Asian Vegetable Research and Development Center and in farmers' fields in southern Taiwan. However, the brittleness of the discolored leaves, occasional upward leaf rolling, and abundance of whiteflies on these plants suggested the involvement of Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV) that belong to the group of whitefly-transmitted, phloem-limited criniviruses (family Closteroviridae). Leaves of symptomatic and healthy plants were collected, and total nucleic acids were extracted from 0.2 g of leaf tissue (1). The total nucleic acids were precipitated by ethanol and dissolved in 160 μl of sterile water. Eight microliters of total nucleic acids were observed on positively charged nylon membranes (Roche Diagnostic GmbH, Roch Applied Science, Germany). Two digoxigenin-labeled riboprobes, transcribed from pTIC8-44 (complementary to the 3'-end region of TICV RNA 1) and pToC 78 (corresponding to the coat protein region of ToCV RNA 2), were used in hybridization tests to detect TICV and ToCV, respectively (2). Six of seventeen symptomatic tom...Continue Reading

Citations

Jul 4, 2019·Molecular Plant Pathology·Elvira Fiallo-Olivé, Jesús Navas-Castillo
Apr 7, 2010·Molecular Plant-microbe Interactions : MPMI·Inge M HanssenBart P H J Thomma
Apr 12, 2019·Pakistan Journal of Biological Sciences : PJBS·Aly Mohamed Mamoun Abdel-SalamRehab A Dawoud
Dec 24, 2005·Archives of Virology·G LozanoJ Navas-Castillo
Jun 1, 2006·Plant Disease·William M Wintermantel, Gail C Wisler
Feb 25, 2020·Journal of Agricultural and Food Chemistry·Huanyu YangBaoan Song
Apr 17, 2020·Journal of Agricultural and Food Chemistry·Leilei RanDeyu Hu

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