Abstract
During December 2003, symptoms were observed in greenhouse tomato plants in Bet Dagan, Israel that resembled those of Tomato chlorosis virus (ToCV), a crinivirus common in the southeastern United States and southern Europe (2,3). Middle-aged leaves showed interveinal chlorosis, while more mature leaves showed more intense interveinal chlorosis with some interveinal bronzing. Symptoms were associated with the presence of Bemisia tabaci, an efficient vector of ToCV. Total nucleic acids were extracted (1) from middle-aged and mature leaves from two symptomatic plants, as well as from healthy tomato, Physalis wrightii infected with ToCV, and Nicotiana benthamiana infected with Tomato infectious chlorosis virus (TICV), another crinivirus that produces identical symptoms on tomato. Extracts were tested using hybridization with probes specific to the coat protein (CP) gene of ToCV and the HSP70h gene of TICV. Hybridization results identified the presence of ToCV in all samples from symptomatic tomato plants and ToCV-infected P. wrightii, but not in those from healthy tomato or TICV-infected N. benthamiana. TICV was only detected in TICV-infected N. benthamiana. Extracts were also subjected to reverse transcription-polymerase chain rea...Continue Reading
Citations
Jul 4, 2019·Molecular Plant Pathology·Elvira Fiallo-Olivé, Jesús Navas-Castillo
Jan 24, 2019·Plant Disease·Ya-Chi KangTsung-Chi Chen
Dec 24, 2005·Archives of Virology·G LozanoJ Navas-Castillo
Nov 13, 2008·Phytopathology·William M WintermantelLaura L Hladky
May 15, 2010·Phytopathology·Elena García-CanoRafael Fernández-Muñoz
Apr 7, 2010·Molecular Plant-microbe Interactions : MPMI·Inge M HanssenBart P H J Thomma
Apr 12, 2019·Pakistan Journal of Biological Sciences : PJBS·Aly Mohamed Mamoun Abdel-SalamRehab A Dawoud
Feb 2, 2018·Frontiers in Plant Science·Xiaobin ShiYong Liu
Jun 1, 2006·Plant Disease·William M Wintermantel, Gail C Wisler
Jun 12, 2018·The Plant Pathology Journal·Ye-Ji LeeHong-Soo Choi
Sep 14, 2010·Journal of Virological Methods·William M Wintermantel, Laura L Hladky
Apr 27, 2019·Journal of Agricultural and Food Chemistry·Dandan XieBaoan Song
Feb 25, 2020·Journal of Agricultural and Food Chemistry·Huanyu YangBaoan Song
Oct 12, 2021·Journal of Agricultural and Food Chemistry·Donghao JiangBaoan Song