First Report of Tomato yellow leaf curl virus Associated with Beans, Phaseolus vulgaris, in Cuba

Plant Disease
Y Martínez ZubiaurD P Maxwell

Abstract

Beans with yellow mosaic and/or leaf crumple symptoms were collected in three fields in the southern area of the province of Havana, Cuba in December 2001 and February 2002. DNA was extracted from the fresh bean leaves of 25 samples (1). Dot blot hybridization was performed at high stringency with a specific probe for Tomato yellow leaf curl virus (TYLCV). The specific probe was prepared by alkaline phosphatase labeling of the polymerase chain reaction (PCR) fragment amplified with primer pair, PTYIRv21/PTYIRc287, containing the intergenic region (IR) of TYLCV, and chemiluminescent hybridization was completed as described by the manufacturer (AlkPhos Direct Labeling and Detection Systems, Amersham Pharmacia Biotech Inc., Piscataway, NJ). Four of the samples had positive hybridization signals. PCR was performed with overlapping primers for TYLCV (2) with the DNA extract from sample 01-44, which gave a positive hybridization signal with the TYLCV probe, and a 2.8-kb fragment was obtained. This fragment was cloned in pGem T-Easy (pBeTY44) and partially sequenced. Greater than 96% nt identity was obtained for the 591 nt of the IR and 504 nt of the N-terminus of the Rep gene with TYLCV (GenBank Accession No. AF260331). Also, PCR was...Continue Reading

Citations

May 17, 2011·Annual Review of Phytopathology·Jesús Navas-CastilloSonia Sánchez-Campos
Nov 28, 2012·Archives of Virology·Elvira Fiallo-OlivéEnrique Moriones
Feb 9, 2017·Archives of Virology·Lidia Chang-SidorchukYamila Martínez-Zubiaur

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