First Report of Wheat dwarf virus in Winter Wheat in Finland

Plant Disease
A Lemmetty, E Huusela-Veistola

Abstract

During June and July of 2004, several diseased plants in winter wheat (Triticum aestivum L.) were reported by agricultural advisers in the southern and southwestern coastal area of Finland. The plants showed extreme dwarfing, various yellowing symptoms, and reduced or no heading. The damage varied considerably. Yield loss estimates in direct-drilled winter wheat fields were approximately 20 to 40% and in worst cases as much as 100%. A few leafhoppers (Psammotettix alienus Dahlb.) were collected from the field with sweep nets and yellow traps. Roots and symptomatic leaves of winter wheat and the leafhoppers were first tested using a commercial polyclonal antibody (DSMZ, Braunschweig, Germany) specific for Wheat dwarf virus (WDV). For the leaf and root samples, routine double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) procedures were used. Five leafhoppers per sample were homogenized with the extraction buffer provided. The homogenate was centrifuged and the extract was evaluated using DAS-ELISA (2). The highest absorbance values were obtained from leafhoppers suspected to be viruliferous. The mean values varied from 1.002 to 1.990 after incubation in the substrate for 2 h. The absorbance values of several le...Continue Reading

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